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Thermo Scientific™ O-Dianisidine 97%
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Formula: C₁₄H₁₆N₂O₂
CAS Number: 119-90-4
Specifications:
| Application | Biochemical Assays | ||
| Storage Temperature | Ambient | ||
| Product Type | Dye | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Analytical grade | Formula | C₁₄H₁₆N₂O₂ |
Thermo Scientific™ O-Dianisidine (97%) is a high-purity aromatic diamine widely used in biochemical, enzymatic, and analytical laboratory applications, particularly in peroxidase-based colorimetric assays. The compound is commonly employed as a chromogenic substrate for peroxidase enzymes, producing a measurable color change in the presence of hydrogen peroxide and peroxidase activity.
O-Dianisidine is frequently used in clinical chemistry, microbiology, and biochemical research to measure enzyme activity such as peroxidase and oxidase reactions. Due to its strong chromogenic properties, it is also applied in histochemical staining, hemoglobin detection, and diagnostic assay development.
This reagent is supplied as a beige to light brown crystalline powder with a purity of ≥96–97%, ensuring reliable performance in research and analytical procedures.
Key Features
- High Purity: ≥97% purity for reliable laboratory performance
- Chromogenic Substrate: Ideal for peroxidase and oxidase enzyme assays
- Consistent Quality: Analytical-grade reagent suitable for research laboratories
- Strong Colorimetric Response: Produces measurable color changes in enzymatic reactions
- Stable Crystalline Form: Powder or crystalline solid suitable for long-term storage
- Versatile Applications: Biochemistry, clinical chemistry, microbiology, and analytical research
Applications
Thermo Scientific O-Dianisidine is widely used in:
- Peroxidase activity assays
- Hydrogen peroxide detection assays
- Enzyme kinetics studies
- Colorimetric biochemical assays
- Hemoglobin detection
- Histochemical staining procedures
- Clinical diagnostic reagent development
- Oxidative enzyme research
- Microbiological enzyme tests
Chemical and Physical Properties
| Property | Specification |
|---|---|
| Product Name | O-Dianisidine |
| Purity | ≥97% |
| Synonyms | 4,4′-Diamino-3,3′-dimethoxybiphenyl; 3,3′-Dimethoxybenzidine |
| CAS Number | 119-90-4 |
| Molecular Formula | C₁₄H₁₆N₂O₂ |
| Molecular Weight | 244.29 g/mol |
| MDL Number | MFCD00008372 |
| EINECS Number | 204-355-4 |
| Appearance | Beige to pink to light brown crystalline powder |
| Melting Point | 134–140 °C |
| Storage Temperature | Ambient |
| Infrared Spectrum | Conforms |
| Assay (HClO₄ Titration) | ≥96% |
Safety Information
| Classification | Details |
|---|---|
| Hazard Class | Irritant |
| Transport Classification | ADR 6.1 (Toxic substances), Packing Group II |
| Handling | Use appropriate personal protective equipment (PPE) |
| Storage | Store in a cool, dry place away from light |
Due to its potential toxicity, this compound should be handled in accordance with laboratory safety guidelines and SDS recommendations.
Ordering Information
| Catalog Number | Packaging | Quantity |
|---|---|---|
| 407890050 | Glass Bottle | 5 g |
| 407890250 | Glass Bottle | 25 g |
Thermo Scientific™ O-Dianisidine 97% is a reliable chromogenic reagent designed for sensitive enzyme detection and biochemical assays. Its high purity, strong colorimetric properties, and compatibility with peroxidase-based analytical systems make it an essential reagent in biochemistry, clinical research, and enzymology laboratories. With consistent quality and dependable performance, O-Dianisidine supports accurate detection and measurement of enzymatic activity across a wide range of research applications.
- Pack Size: 5g 25g