Thermo Scientific™ Malachite green oxalate, pure, high purity biological stain

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Specifications:
Application Microbiology
Storage Temperature Ambient
Product Type Biological Stain Forms Powder
Product Brand Thermo Fisher Scientific™, thermo Scientific
Product Grade Microbiology grade Formula C₅₂H₅₄N₄O₁₂

The Thermo Scientific™ Malachite Green Oxalate is a high-purity biological stain widely used in microbiology, histology, and biochemical research. Known as Basic Green 4 (C.I. 42000), this compound is supplied in crystalline powder form, with high absorbance and consistency, making it suitable for applications such as endospore staining, parasite detection, and phosphatase activity assays.

This product is manufactured to exacting purity standards, ensuring reproducibility in both research and diagnostic staining protocols.

Chemical Information

  • Chemical Name: Malachite Green Oxalate
  • Synonyms: Basic Green 4, C.I. 42000, bis(bis4-(dimethylamino)phenylmethylium) oxalic acid dihydrogen oxalate
  • Molecular Formula: C₅₂H₅₄N₄O₁₂
  • Molecular Weight: 927.02 g/mol
  • CAS Number: 2437-29-8
  • EC Number: 219-441-7

Key Features

  • High-purity biological stain for reliable staining performance.
  • UV λmax: 615–619 nm (water).
  • Specific absorbance (A 1%/1 cm): ≥1800.
  • Absorption Ratio P-15/P+15: 0.9–1.15.
  • Appearance: Green to dark green crystalline powder or crystals.
  • Excellent reproducibility for staining and analytical use.
  • Supplied in sealed glass bottle packaging.

Applications

  • Microbiology: Endospore staining, acid-fast bacillus differentiation.
  • Histology: Counterstaining and cytological staining protocols.
  • Biochemistry: Colorimetric detection of phosphate in malachite green assays.
  • Parasitology: Staining protozoa and helminth eggs in samples.
  • Environmental studies: Used in dye and pollutant analysis.

Specifications

PropertyValue
Catalog Number229780250
PackagingGlass bottle
Available Quantity25 g
Price£19.30 (Online Exclusive, £22.70 standard)
UV λmax615–619 nm (water)
Specific Absorbance≥1800
P-15/P+15 Absorption Ratio0.9–1.15
Loss on Drying≤10% (at 105 °C)
FormCrystalline powder
ColorGreen to dark green
Infrared SpectrumConforms


The Thermo Scientific™ Malachite Green Oxalate is a reliable, high-purity biological stain for research applications in microbiology, histology, and biochemistry. With consistent specifications, glass bottle packaging, and strong spectral properties, it provides reproducibility and accuracy for both routine and advanced staining protocols.

  • Pack Size:   100g 500g 25g
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