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Thermo Scientific™ L-Leucine-p-nitroanilide (≥99%)
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Specifications:
| Application | Analytical Chemistry, Colorimetric Assay | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Laboratory Chemical | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Analytical grade | Formula | C₁₂H₁₇N₃O₃ |
The Thermo Scientific™ L-Leucine-p-nitroanilide (Leu-pNA) is a high-purity synthetic chromogenic substrate widely used in enzymatic activity assays, particularly for proteases and peptidases. With a purity of ≥99%, this compound delivers reliable and reproducible performance in biochemical and molecular biology workflows.
L-Leucine-p-nitroanilide is designed to release p-nitroaniline (pNA) upon enzymatic cleavage, producing a measurable yellow chromophore that can be quantified spectrophotometrically. This makes it highly suitable for kinetic enzyme studies, inhibitor screening, and substrate specificity investigations in research and quality control laboratories.
Manufactured to high analytical standards, this product is ideal for use in life sciences research, pharmaceutical development, and enzyme characterization studies.
Key Features
- High purity (≥99%) for accurate and reproducible assay results
- Chromogenic substrate that releases p-nitroaniline (pNA) upon enzymatic cleavage
- Ideal for protease and peptidase activity assays
- Suitable for spectrophotometric detection methods
- Reliable product from Thermo Scientific™
- Available in multiple pack sizes for research flexibility
Applications
- Protease and peptidase enzyme activity assays
- Kinetic studies and enzyme characterization
- Drug discovery and inhibitor screening
- Biochemical and molecular biology research
- Quality control in pharmaceutical and biotech laboratories
- Substrate specificity testing
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | L-Leucine-p-nitroanilide |
| Synonyms | H-Leu-pNA; (S)-2-Amino-4-methyl-N-(4-nitrophenyl)pentanamide |
| CAS Number | 4178-93-2 |
| Molecular Formula | C₁₂H₁₇N₃O₃ |
| Molecular Weight | 251.29 g/mol |
| Purity | ≥99% |
| Format | Solid |
| Melting Point | 89–90 °C |
| Storage Temperature | 2–8 °C |
| EINECS Number | 224-047-3 |
| MDL Number | MFCD00063681 |
| Beilstein Reference | 2218433 |
| Pack Sizes | 1 g, 5 g |
Advantages
The Thermo Scientific™ L-Leucine-p-nitroanilide offers high sensitivity and ease of detection due to its chromogenic nature, enabling straightforward monitoring of enzymatic reactions via absorbance measurements. Its high purity and consistent quality ensure reproducibility across experiments, making it a dependable reagent for both routine assays and advanced research applications.
The compound’s compatibility with standard spectrophotometric methods simplifies workflow integration, reducing the need for complex detection systems.
The Thermo Scientific™ L-Leucine-p-nitroanilide (≥99%) is a reliable and high-performance chromogenic substrate for enzyme assays. With its proven analytical quality, ease of use, and strong applicability in protease research, it is an essential reagent for laboratories focused on biochemical analysis, drug discovery, and enzymology studies.
- Pack Size: 1 g 5 g