Sigma-Aldrich Neuraminidase from Vibrio cholerae Type III

Sigma-Aldrich Neuraminidase from Vibrio cholerae, Type III is a high-quality, purified enzyme preparation supplied as a buffered aqueous solution. This enzyme belongs to the glycoside hydrolase family (EC 3.2.1.18) and catalyzes the hydrolysis of terminal sialic acid residues from glycoproteins, glycolipids, and oligosaccharides.

With a specific activity of 1–5 units/mg protein (Lowry, NAN-lactose substrate) and molecular weight of ~83 kDa, this preparation is widely used as a cell-surface probe, in substrate specificity studies, and in diagnostic assay development.

Key Features

• Type III enzyme preparation from V. cholerae.
• Buffered aqueous solution, pH 5.5 with 0.15 M NaCl and 4 mM CaCl₂.
• Chromatographically purified and preservative-free.
• Molecular weight: ~83 kDa.
• Specific activity: 1–5 units/mg protein (Lowry, NAN-lactose).
• Storage temperature: 2–8 °C for stability.
• Foreign activity present: Protease and NAN-aldolase.

Specifications

Applications

• Diagnostic assay manufacturing – for enzyme-linked assays and research kits.
• Glycoconjugate mapping – used as a probe for analyzing cell-surface glycoconjugate distribution.
• Substrate specificity studies – research into enzyme-substrate interactions.
• Pathogenesis research – study of cholera toxin uptake and infection mechanisms.
• Fluorometric assays – employed in neuraminidase substrate evaluation.

Biochemical/Physiological Actions

• Promotes cholera toxin infection by binding the toxin and aiding its uptake by susceptible cells.
• Hydrolyzes terminal sialic acid residues from glycoproteins and glycolipids, influencing cell–pathogen interactions.
• Valuable in glycobiology for studying the role of sialic acid in host–pathogen recognition.

The Sigma-Aldrich Neuraminidase from Vibrio cholerae, Type III is a purified, reliable enzyme reagent for research in glycobiology, pathogen-host interaction studies, diagnostic assay development, and substrate specificity research. With high activity and consistency, this preparation is widely trusted in academic, clinical, and pharmaceutical laboratories.