Sigma-Aldrich L-Tyrosine ≥98% (HPLC)

L-Tyrosine is a non-essential, proteogenic amino acid with a polar side chain that plays a key role in biological processes such as protein synthesis, signal transduction, and neurotransmitter production. It is a precursor for dopamine and other catecholamines, making it crucial in neuroscience and biochemical research.

Chemical Properties:

Physical Properties:

Applications:

1. Biochemical Research:
   • Used to prepare solutions for tyrosinase assays.
   • Key substrate in the assay of pepsin activity.
2. Signal Transduction Studies:
   • Investigated as a phosphate group receiver in kinase-mediated signal transduction pathways.
3. Neurochemical Studies:
   • Precursor of dopamine and other catecholamines, essential for studying neurotransmitter synthesis.
4. Protein Studies:
   • Incorporated into studies on protein biosynthesis and structure.

Biochemical/Physiological Actions:

• Role in Signal Transduction:
  L-Tyrosine participates in cellular communication as a substrate for phosphorylation by kinases, influencing signaling pathways.
• Precursor for Catecholamines:
  It serves as a starting material for the biosynthesis of dopamine, norepinephrine, and epinephrine.
• Protein Building Block:
  As a proteogenic amino acid, L-Tyrosine is directly incorporated into proteins, influencing their function and stability.

Preparation and Handling:

• Form:
  Powder form for easy handling and dissolution.
• Storage:
  Store in a cool, dry place at ambient temperature to maintain stability.

Advantages:

• High Purity:
  Assay of ≥98% (HPLC) ensures reliability in research applications.
• Versatile Applications:
  Suitable for enzymatic assays, signal transduction studies, and neurotransmitter research.
• Stable Formulation:
  Powder form with long shelf-life under appropriate storage conditions.

Citations and Use Cases:

1. Tyrosinase Assays:
   • L-Tyrosine is utilized as a substrate to evaluate enzymatic activity. (Reference 1)
2. Pepsin Activity Assays:
   • Used to determine pepsin activity in biochemical studies. (Reference 2)
3. Signal Transduction Studies:
   • Explored for its role in kinase-mediated phosphorylation. (Reference 3)
4. Dopamine Synthesis Studies:
   • Acts as a precursor in the biosynthesis of dopamine and related compounds. (Reference 4)

Sigma-Aldrich L-Tyrosine is a high-quality reagent suitable for diverse applications in biochemistry, neuroscience, and molecular biology. Its role as a substrate in enzymatic assays and its significance in neurotransmitter biosynthesis make it an indispensable tool for research. With a purity level of ≥98%, it offers reliability and precision for scientific studies.