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SERVA Acrylamide/Bisacrylamide (29:1) 30% in aqueous solution
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Specifications:
| Application | Gel Electrophoresis | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | SERVA Electrophoresis GmbH | ||
| Product Grade | Molecular Biology | ||
The SERVA Acrylamide/Bis Solution, 29:1 (30% w/v), 3.3% C, is a ready-to-use electrophoresis-grade solution designed for the preparation of polyacrylamide gels (PAGE). This solution is optimized for the separation of proteins/peptides <30 kDa, DNA oligonucleotides, and long-run DNA sequencing applications.
By eliminating the need to handle toxic acrylamide powders, this solution enhances safety while providing high reproducibility and resolution for electrophoretic separations. Each batch undergoes rigorous gel electrophoresis QC testing to ensure reliable performance in protein and nucleic acid analysis.
Key Features
- Pre-mixed acrylamide:bisacrylamide solution (29:1 ratio, 30% w/v).
- 3.3% crosslinker concentration (C) for protein/peptide gels (<30 kDa) and DNA applications.
- Free from hazardous acrylamide dust exposure.
- Made from ultra-pure acrylamide with very low acrylic acid content (<0.03%).
- Ensures sharp band resolution in PAGE and DNA sequencing.
- Batch-tested by gel electrophoresis QC for reproducibility.
- Conveniently supplied in 500 mL and 1 L bottles, plus multipacks.
Specifications
| Parameter | Details |
|---|---|
| Composition | Acrylamide:N,N′-methylene bisacrylamide (29:1) |
| Concentration | 30% (w/v) |
| %C (crosslinker) | 3.3% |
| pH (5% solution) | 6.0 – 8.0 |
| Absorbance at 290 nm (5%) | <0.7 |
| Conductivity (µS, 5%) | <100 |
| Free acrylic acid | <0.03% |
| Storage Temperature | +2 °C to +8 °C |
| Hazard Classification | Carc. 1B, Muta. 1B, Repr. 2 |
| UN Number | 3426 |
| ADR/IATA | 6.1 III (toxic substance) |
| HS Code | 38229000 |
| WGK | 3 (severe hazard to waters) |
Applications
- SDS-PAGE and native PAGE for proteins and peptides <30 kDa.
- High-resolution DNA sequencing gels.
- Electrophoretic separation of oligonucleotides.
- Research laboratories in proteomics, genomics, and molecular biology.
Safety Information
- Classified as toxic, carcinogenic, mutagenic, and reprotoxic (CMR).
- Hazard Statements: H302, H312, H315, H317, H319, H340, H350, H361f, H372.
- Precautions: P201, P264, P280 (protective clothing, gloves, eye/face protection).
- Handle under fume hood and store at +2 to +8 °C.
- UN 3426, ADR/IATA Class 6.1 III – toxic substance regulations apply.
The SERVA Acrylamide/Bis Solution, 29:1 (30% w/v), 3.3% C, offers a safe, reliable, and ready-to-use solution for preparing high-resolution polyacrylamide gels. By providing consistent performance, reduced handling risk, and compliance with QC testing, it is an essential reagent for laboratories conducting protein electrophoresis, DNA sequencing, and nucleic acid separations.
- Pack Size: 1L 500 mL 4 x 500 mL