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Virplex® Bordetella RealTime PCR Kit, 4-Plex, 48 Rxn
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Targets: Bordetella pertussis (multiplex, 4-target detection) + RNAse P internal control
Specifications:
| Application | qPCR, Molecular Diagnostics | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Lyophilized |
| Product Brand | VIRCELL | ||
| Product Grade | Molecular Biology | ||
The Virplex® Bordetella RealTime PCR Kit is a multiplex real-time RT-PCR assay designed for the detection of Bordetella pertussis nucleic acids in human nasopharyngeal swab and lavage samples. This CE-IVDR certified kit provides laboratories with a rapid, sensitive, and specific molecular tool for the diagnosis of whooping cough (pertussis).
Key Features
- 4-target multiplex PCR – one single reaction tube per sample.
- Specific detection of Bordetella pertussis in respiratory specimens.
- Endogenous human RNAse P control – ensures monitoring of inhibitors, nucleic acid integrity, and proper amplification set-up.
- Compatible with 4-channel qPCR systems – streamlined for clinical use.
- Fast turnaround – results in under 2 hours.
- Lyophilized reagents – master mix and positive control included, improving stability and reducing transportation costs.
- CE-IVDR certified – compliant with European diagnostic regulations.
Applications
- Molecular detection of Bordetella pertussis in suspected pertussis (whooping cough) cases.
- Diagnostic testing of nasopharyngeal swabs and lavage samples.
- Clinical and epidemiological surveillance of pertussis outbreaks.
- Research applications in respiratory pathogen detection.
- Training and validation of molecular diagnostic methods in public health laboratories.
Product Details
| Property | Value |
|---|---|
| Product Name | Virplex® Bordetella RealTime PCR Kit |
| Reference | RTPCR030-LP |
| Nº Tests | 48 |
| Class | CE0123 IVDR |
| Sample Type | Human nasopharyngeal swabs and lavage specimens |
| Targets | Bordetella pertussis (multiplex, 4-target detection) + RNAse P internal control |
| PCR Cycler Compatibility | 4-channel systems |
| Time to Results | < 2 hours |
| Reagents | Lyophilized master mix, positive control, negative control |
| Format | Ready-to-use lyophilized multiplex assay |
| Target | Gene/Marker | Role in Assay | Clinical Relevance |
|---|
| Bordetella pertussis (Primary marker) | IS481 insertion sequence | Highly sensitive detection of B. pertussis | Detects pertussis (whooping cough); present in high copy numbers in B. pertussis genome |
| Bordetella pertussis (Confirmatory marker) | ptxA / ptxP (Pertussis toxin gene promoter region) | Specific confirmation of B. pertussis infection | Confirms true B. pertussis cases; rules out false positives from IS481 cross-reactivity |
| Bordetella pertussis (Species-specific marker) | recA or equivalent housekeeping gene | Discriminates B. pertussis from related Bordetella spp. | Prevents misidentification with B. holmesii or B. bronchiseptica |
| Internal Control (IC) | Human RNAse P gene | Monitors amplification, sample integrity, and inhibition | Validates proper sample collection and ensures reliable test results |
Related Products
- AMPLIRUN® Bordetella DNA Control – external quality control for assay validation.
The Virplex® Bordetella RealTime PCR Kit provides a fast, accurate, and stable molecular solution for the detection of Bordetella pertussis. With its multiplex format, internal control system, lyophilized reagents, and CE-IVDR certification, it is an essential tool for clinical diagnostics, outbreak surveillance, and laboratory quality assurance in respiratory pathogen detection.