ROTH Pepsin ≥2.0 FIP-U/mg, Ph. Eur., Lyophilized

ROTH Pepsin ≥2.0 FIP-U/mg, Ph. Eur., lyophilized is a high-activity acidic endopeptidase widely used in biochemical, analytical, and food safety applications. Derived from the inactive precursor pepsinogen, pepsin is a proteolytic enzyme that cleaves proteins preferentially at the carboxyl groups of aromatic amino acids, such as phenylalanine and tyrosine.

This high-purity enzyme preparation is particularly suited for detection of Trichinella (trichina) in meat, as well as for controlled protein digestion in laboratory workflows. The lyophilized format ensures stability during storage and convenient reconstitution for use.

Key Features

• High enzymatic activity: ≥2.0 FIP-U/mg
• Complies with European Pharmacopoeia (Ph. Eur.) standards
• Supplied as lyophilized powder for enhanced stability
• Efficient protein digestion under strongly acidic conditions (pH 1–2)
• Well soluble in water and stable in acidic solutions (pH ≤5.5)
• Suitable for food safety testing and biochemical applications
• Reliable performance for regulated analytical workflows

Applications

• Detection of Trichinella in meat samples
• Protein digestion and hydrolysis
• Biochemical and enzymatic studies
• Food safety and quality control testing
• Sample preparation for protein analysis
• Enzymatic digestion in research and industrial laboratories

Biochemical & Functional Properties

• Enzyme type: Acidic endopeptidase
• Substrate specificity: Cleaves proteins at aromatic amino acids
• pH optimum: 1.0 – 2.0
• Isoelectric point: 1.0
• Stable in aqueous solutions up to pH 5.5
• Irreversibly inactivated at alkaline pH (>6.0)
• Stable up to 60°C and in denaturing agents such as urea and guanidine

Unit Definition

1 FIP unit (≈ Ph. Eur. unit) corresponds to the enzymatic activity that, under defined conditions, produces a degree of hemoglobin decomposition equivalent to the color formed by 1 µmol of tyrosine.

• 2000 FIP-U ≈ 1:10,000 NF-U
• 2000 FIP-U ≈ 1:12,500 BP-U

Technical Specifications

Product Variants

The ROTH Pepsin ≥2.0 FIP-U/mg, Ph. Eur. is a high-quality enzymatic reagent designed for reliable protein digestion and regulated analytical applications. Its high activity, well-defined biochemical properties, and compliance with pharmacopoeial standards make it particularly suitable for food safety testing, including trichina detection, and advanced laboratory workflows.