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Invitrogen™ Resorufin β-D-Galactopyranoside, 25mg
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Specifications:
| Application | Cell Analysis | ||
| Storage Temperature | -5 to -30°C | ||
| Product Type | Molecular Biology Reagent | Forms | Solid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | Formula | C₁₈H₁₇NO₈ |
Fluorogenic β-galactosidase substrate for sensitive enzyme activity detection
Invitrogen™ Resorufin β-D-Galactopyranoside is a fluorogenic substrate for β-galactosidase (β-gal), widely used in enzyme assays, reporter gene detection, and cell biology research. Upon enzymatic hydrolysis by β-galactosidase, it yields resorufin, a bright red-fluorescent product with absorption and emission maxima at ~571/585 nm.
The long-wavelength fluorescence minimizes cellular autofluorescence and spectral overlap with green and blue fluorophores, making it suitable for multiplexed fluorescence assays. Resorufin β-D-galactopyranoside is especially valuable in LacZ reporter gene assays for both in vitro and live-cell applications.
Key Features
- Fluorogenic β-galactosidase substrate for sensitive detection
- Bright red fluorescence with excitation/emission at 571/585 nm
- Low background due to minimal interference from autofluorescence
- Multiplex-compatible with green and blue fluorophores (e.g., FITC, GFP)
- Suitable for live-cell imaging, plate-based assays, and flow cytometry
Applications
- LacZ reporter gene assays in molecular biology
- Enzyme activity detection in cell-based and biochemical assays
- Multiparameter fluorescence analysis in flow cytometry
- Live-cell β-galactosidase imaging with minimal spectral overlap
- Screening assays in drug discovery and gene expression studies
Specifications
| Parameter | Details |
|---|---|
| Catalog Number | R1159 |
| Chemical Name | Resorufin β-D-Galactopyranoside |
| Quantity | 25 mg |
| Substrate Type | β-galactosidase substrate |
| Excitation/Emission | ~571/585 nm |
| Form | Solid |
| Detection Method | Fluorescence |
| Substrate Properties | Chemical substrate |
| Storage Conditions | –5°C to –30°C, protect from light |
| Shipping Conditions | Room temperature |
| Regulatory Status | Research Use Only (RUO) |
Invitrogen™ Resorufin β-D-Galactopyranoside is an ideal choice for researchers requiring sensitive, multiplex-compatible β-galactosidase detection in both biochemical and live-cell assays. Its red-shifted fluorescence improves signal clarity and enables high-quality data acquisition in complex experimental setups.