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Sigma-Aldrich™ TWEEN® 80 for Molecular Biology, Syrup, 100 mL
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Specifications:
| Application | molecular biology, Protein Purification | ||
| Storage Temperature | Ambient | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Sigma-Aldrich | ||
| Product Grade | Molecular Biology | ||
Sigma-Aldrich™ TWEEN® 80 for Molecular Biology is a molecular biology–tested non-ionic detergent supplied as a syrup. Also known as Polysorbate 80, it is widely used as a surfactant, emulsifier, and stabilising agent in biological and biochemical workflows.
This grade is tested for molecular biology suitability, with DNase, RNase, nickase, and exonuclease activities not detected, making it appropriate for sensitive nucleic acid and protein-related laboratory applications. TWEEN® 80 is commonly used for selective protein extraction, membrane-related workflows, and isolation of nuclei from mammalian cell lines.
Key Features
- Molecular biology grade TWEEN® 80 / Polysorbate 80
- Non-ionic detergent supplied as a syrup
- Suitable for biological and biochemical applications
- Molecular biology tested
- DNase, RNase, nickase, and exonuclease activity not detected
- Useful as a stabiliser, emulsifier, and surfactant
- HLB value of 15
- CMC: 0.012 mM
- Average molecular weight: 1310
- Density: 1.06 g/mL at 25°C
- Supplied in 100 mL pack size
Applications
- Selective protein extraction
- Isolation of nuclei from mammalian cell lines
- Molecular biology workflows
- Biochemical reagent preparation
- Protein stabilisation
- Emulsion preparation
- Membrane and cell-based studies
- Laboratory surfactant applications
- Detergent-based sample preparation
Product Specifications
| Parameter | Specification |
|---|---|
| Brand | Sigma-Aldrich™ |
| Product Name | TWEEN® 80 for Molecular Biology |
| SKU | P5188-100ML |
| Pack Size | 100 mL |
| CAS Number | 9005-65-6 |
| Grade | Molecular Biology |
| Quality Level | 200 |
| Description | Non-ionic |
| Form | Syrup |
| Average Molecular Weight | 1310 |
| Composition | Oleic acid ≥58.0%, balance primarily linoleic, palmitic, and stearic acids |
| CMC | 0.012 mM |
| Cloud Point | 65°C |
| Density | 1.06 g/mL at 25°C |
| HLB | 15 |
| Suitability | Molecular biology tested |
| Foreign Activity | DNase, RNase, nickase, and exonuclease not detected |
| UNSPSC Code | 12161900 |
| NACRES | NA.21 |
| MDL Number | MFCD00082107 |
Synonyms
- TWEEN® 80
- Polysorbate 80
- POE (20) sorbitan monooleate
- Polyethylene glycol sorbitan monooleate
- Polyoxyethylenesorbitan monooleate
Sigma-Aldrich™ TWEEN® 80 for Molecular Biology, P5188-100ML is a dependable non-ionic detergent for sensitive molecular biology and biochemical workflows. Its molecular biology-tested quality, absence of detectable nuclease activity, and usefulness in protein extraction, nuclei isolation, and surfactant-based sample preparation make it a valuable reagent for research and routine laboratory applications.