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Sigma-Aldrich™ Pseudomonas Exotoxin A from Pseudomonas aeruginosa, 0.5mg
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protein synthesis inhibitor
Specifications:
| Application | molecular biology, cancer research, Cell Biology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Enzymes and Substrates, Bioactive small molecules | Forms | lyophilized powder |
| Product Brand | Sigma-Aldrich | ||
| Product Grade | Molecular Biology | ||
Sigma-Aldrich™ Pseudomonas Exotoxin A is a highly purified protein synthesis inhibitor derived from Pseudomonas aeruginosa. Supplied as a lyophilized powder, this toxin is widely used in molecular biology, cell biology, and cancer research for studying protein synthesis inhibition and ADP-ribosylation mechanisms.
Pseudomonas exotoxin A belongs to the family of mono-ADP-ribosyltransferases and exerts its biological effect by modifying elongation factor 2 (EF-2), leading to inhibition of protein synthesis. Due to its potent cytotoxic activity, it is also utilized in anticancer research and targeted toxin studies.
Key Features
- Purified Pseudomonas exotoxin A from P. aeruginosa
- Functions as a protein synthesis inhibitor
- Member of mono-ADP-ribosyltransferase enzyme family
- Supplied as stable lyophilized powder
- Suitable for cell biology and cancer research applications
- Quality Level 200 for research consistency
Applications
- Protein synthesis inhibition studies
- ADP-ribosylation research
- Cancer and cytotoxicity studies
- Cell signaling and translation research
- Targeted toxin and immunotoxin development
- Molecular and biochemical pathway analysis
Technical Specifications
| Parameter | Description |
|---|---|
| Product Name | Pseudomonas Exotoxin A |
| Source | Pseudomonas aeruginosa |
| CAS Number | 91262-95-2 |
| Form | Lyophilized powder |
| Quality Level | 200 |
| Composition | Protein (~30%, E1%/280) |
| Buffer Components | Tris buffer, EDTA, NaCl, lactose |
| Storage Conditions | 2–8°C |
| MDL Number | MFCD00132134 |
| UNSPSC Code | 12352200 |
| NACRES | NA.77 |
Ordering Information
| Pack Size | SKU |
|---|---|
| 0.5 mg | P0184-.5MG |
Biochemical Properties
- Catalyzes transfer of ADP-ribose from NAD⁺ to diphthamide residue on EF-2
- Inhibits protein synthesis via elongation factor 2 inactivation
- Exhibits high cytotoxicity in cell lines and animal models
- Widely used as a tool for studying translational control mechanisms