The ONT MinION™ Mk1D Package is a compact, high-performance portable DNA sequencing system designed to deliver real-time genomic analysis in laboratories, field environments, and mobile research settings. The MinION Mk1D is a palm-sized nanopore sequencer that connects directly to modern laptops through a USB-C interface, enabling researchers to perform advanced sequencing workflows anywhere.

Building on the success of the previous MinION Mk1B, the Mk1D introduces improved thermal regulation, enhancing sequencing stability and performance across a wider range of environmental conditions. This makes the device suitable for use in laboratories, field research stations, hospitals, and remote locations.

Using Oxford Nanopore’s nanopore sequencing technology, the MinION Mk1D enables real-time sequencing and analysis of DNA or RNA molecules. As DNA molecules pass through nanopores embedded in sequencing flow cells, changes in electrical current are detected and converted into nucleotide sequences by base-calling software.

The MinION Mk1D is compatible with MinION Flow Cells and Flongle Flow Cells, allowing researchers to scale experiments from small targeted runs to high-throughput genomic sequencing.

Key Features

Ultra-Portable Sequencing Platform

• Palm-sized nanopore sequencer
• Powered via USB-C connection
• Designed for mobile and field sequencing

Real-Time DNA Sequencing

• Sequencing data generated instantly during runs
• Immediate access to sequencing reads for rapid analysis
• Runs can be stopped once sufficient data is collected

Improved Thermal Regulation

• Enhanced temperature management improves sequencing stability
• Supports reliable sequencing in diverse environmental conditions

Easy Device Monitoring

• Integrated status LEDs provide clear device feedback
• Simplifies monitoring during sequencing runs

Flexible Flow Cell Compatibility

• Compatible with:
  • MinION Flow Cells
  • Flongle Flow Cells

This allows flexible scaling of sequencing experiments depending on throughput requirements.

Nanopore Sequencing Technology

The MinION Mk1D uses Oxford Nanopore’s proprietary nanopore sequencing technology:

1. DNA molecules pass through nanopores embedded in a sequencing membrane.
2. Each nucleotide causes a unique change in electrical current.
3. The electrical signal is translated into DNA sequence data using base-calling algorithms.
4. Sequence reads are generated in real time, enabling immediate analysis.

This approach allows:

• Long-read sequencing
• Detection of structural variants
• Direct epigenetic modification detection

Applications

The MinION Mk1D sequencing system is widely used in:

Genomics Research

• Whole genome sequencing
• Structural variant detection
• Genome assembly

Metagenomics

• Microbial community profiling
• Environmental DNA analysis
• Microbiome research

Clinical and Public Health

• Pathogen identification
• Outbreak surveillance
• Antimicrobial resistance studies

Field Sequencing

• Wildlife genomics
• Agricultural genomics
• Environmental monitoring

Education and Research

• Academic genomics studies
• Training in sequencing technologies

Package Contents

The MinION Mk1D Package includes:

• 1 × MinION Mk1D sequencing device
• 1 × 12-month standard support
• 5 × MinION/GridION Flow Cells
• 1 × Rapid Barcoding Kit 24
• 1 × Flow Cell Wash Kit

This bundle provides researchers with the essential components needed to start sequencing immediately.

Device Compatibility

Flow Cells

• MinION Flow Cells
• Flongle Flow Cells

Sequencing Software

• MinKNOW software for device control and run monitoring
• Compatible with ONT base-calling and analysis pipelines

Advantages

• Portable sequencing platform
• Real-time data generation
• Flexible throughput using multiple flow cell options
• Easy setup and operation
• Suitable for both laboratory and field environments

The Oxford Nanopore MinION Mk1D Package provides a powerful and portable sequencing solution for modern genomics research. Its compact design, real-time sequencing capability, and compatibility with multiple flow cell formats allow researchers to perform advanced genomic analysis anywhere. With improved thermal regulation, intuitive device monitoring, and included sequencing consumables, the MinION Mk1D package enables laboratories and field researchers to rapidly generate high-quality sequencing data across a wide range of applications.