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MCE XTT Sodium Salt ≥99.85%
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Colorimetric Cell Viability Reagent for Metabolic Activity and Cytotoxicity Assays
Specifications:
| Application | Colorimetric Assay | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Solid |
| Product Brand | MedChem Express | ||
| Product Grade | Molecular Biology | Formula | C₂₂H₁₇N₇O₁₃S₂Na |
MCE XTT Sodium Salt is a high-purity tetrazolium-based reagent used for the quantitative assessment of cell viability, proliferation, and cytotoxicity.
This assay is based on the reduction of XTT by metabolically active cells into a water-soluble orange formazan product, enabling direct measurement without additional solubilization steps.
Due to its sensitivity and ease of use, XTT is widely applied in cell biology, drug screening, and toxicology studies, offering a reliable alternative to traditional tetrazolium assays.
Key Features and Benefits
Direct Colorimetric Detection
- Produces a water-soluble formazan dye, eliminating the need for solubilization steps
- Enables straightforward absorbance measurement
High Sensitivity for Cell Viability
- Detects metabolic activity based on cellular redox potential
- Suitable for low cell density and subtle viability changes
Convenient and Efficient Workflow
- Simple addition to cell culture systems
- Compatible with standard 96-well plate formats
Flexible Solubility Options
- Soluble in water and DMSO
- Supports preparation of stock solutions for various applications
High Purity and Reproducibility
- ≥99.85% purity ensures consistent assay performance
- Reliable results for research and screening workflows
Applications
- Cell viability and proliferation assays
- Cytotoxicity and drug screening studies
- Metabolic activity measurement
- Cancer and immunology research
- High-throughput screening (HTS) assays
- Cell-based assay development
Assay Principle
XTT is reduced by mitochondrial enzymes in metabolically active cells:
- Viable cells convert XTT into an orange formazan product
- The amount of formazan produced is proportional to the number of viable cells
- Absorbance is measured at 450 nm (reference: 650 nm)
- Results are calculated relative to control samples
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | XTT Sodium Salt |
| Purity | ≥99.85% |
| CAS Number | 111072-31-2 |
| Molecular Formula | C₂₂H₁₆N₇NaO₁₃S₂ |
| Molecular Weight | 673.52 g/mol |
| Appearance | Light yellow to yellow solid |
| Solubility | Water: up to 10 mg/mL; DMSO: up to 40 mg/mL |
| Detection Wavelength | 450 nm (reference 650 nm) |
Available Formats
| Product Code | Description |
|---|---|
| HY-122131-1ML | 10 mM solution in DMSO (1 mL) |
| HY-122131-5MG | 5 mg |
| HY-122131-10MG | 10 mg |
| HY-122131-25MG | 25 mg |
| HY-122131-50MG | 50 mg |
| HY-122131-100MG | 100 mg |
Storage and Stability
- Store solid at -20°C, protected from moisture
-
Store solutions:
- -80°C (up to 6 months)
- -20°C (up to 1 month)
- Protect from light to maintain stability
Preparation and Handling
- Prepare XTT solution at 1 mg/mL in culture medium
- Use in combination with electron coupling reagent (e.g., PMS)
- Prepare fresh solutions for optimal performance
- Avoid prolonged incubation to minimize background signal
Unique Advantages
- No solubilization step required compared to MTT assays
- High sensitivity and reproducibility
- Compatible with high-throughput screening formats
- Simple and rapid assay workflow
- Reliable performance for diverse cell types
MCE XTT Sodium Salt ≥99.85% is a robust and efficient reagent for evaluating cell viability and metabolic activity. Its ease of use, high sensitivity, and compatibility with standard laboratory workflows make it an essential tool for modern cell-based research and drug discovery applications.
- Pack Size: 5 mg 25 mg 10 mM x 1 mL in DMSO 50 mg 100 mg 10 mg