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Specifications:
| Application | Colorimetric Assay | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | MedChem Express | ||
| Product Grade | Cell Culture | ||
The MCE Cell Counting Kit-8 (CCK-8) is a highly sensitive, non-radioactive colorimetric assay kit designed for the quantitative determination of cell viability, proliferation, and cytotoxicity. Based on the WST-8 assay principle, this kit provides a simple, accurate, and high-throughput solution for evaluating cell metabolic activity in response to various treatments.
CCK-8 is widely used in drug discovery, cancer research, toxicology, and cell biology studies, offering superior sensitivity and convenience compared to traditional assays such as MTT. Its ready-to-use format eliminates the need for additional solubilization steps, making it ideal for routine laboratory workflows.
Key Features
-
High Sensitivity & Accuracy
Detects subtle changes in cell viability through dehydrogenase activity. -
Simple One-Step Protocol
No washing or solubilization steps required. -
Water-Soluble Formazan Product
Eliminates the need for additional dissolution steps (unlike MTT assays). -
Non-Radioactive & Safe
Suitable for routine laboratory use. -
High Throughput Compatibility
Ideal for 96-well and 384-well plate formats. -
Wide Application Range
Applicable to proliferation, cytotoxicity, and growth inhibition studies.
Applications
Cell Biology & Research
- Cell viability assessment
- Cell proliferation studies
- Growth inhibition analysis
Drug Discovery & Development
- Cytotoxicity screening of anticancer drugs
- Compound efficacy testing
- Dose-response studies
Toxicology
- Evaluation of toxic effects of chemicals
- Environmental and pharmaceutical toxicity testing
Molecular & Biomedical Research
- Cytokine-induced proliferation studies
- Functional assays in cell signaling research
Assay Principle
The CCK-8 assay is based on the reduction of WST-8 by cellular dehydrogenases in viable cells:
- WST-8 is reduced by mitochondrial dehydrogenases
- Produces a water-soluble orange formazan dye
- The amount of formazan is directly proportional to the number of living cells
- Measured by absorbance (OD) at ~450 nm
Technical Specifications
| Parameter | Specification |
|---|---|
| Assay Type | Colorimetric (WST-8 based) |
| Detection Method | Absorbance (OD ~450 nm) |
| Applications | Cell viability, proliferation, cytotoxicity |
| Format | Liquid reagent |
| Storage | 4°C (protected from light) |
| Shelf Life | Up to 2 years |
Kit Sizes / Ordering Information
| Catalog Number | Product Name | Package |
|---|---|---|
| HY-K0301-1 mL | Cell Counting Kit-8 | 1 mL |
| HY-K0301-5 mL | Cell Counting Kit-8 | 5 mL |
| HY-K0301-30 mL | Cell Counting Kit-8 | 5 mL × 6 |
| HY-K0301-120 mL | Cell Counting Kit-8 | 5 mL × 24 |
Important Usage Notes
- Assay measures dehydrogenase activity, which may be affected by certain compounds
- Reducing agents may interfere with results
- Avoid bubbles during plate reading
- Incubation time varies depending on cell type and density
- High turbidity samples may require background correction
Unique Advantages
-
Superior to Traditional MTT Assays
No solubilization step required, saving time and reducing variability. -
High Sensitivity for Low Cell Numbers
Suitable for scarce or slow-growing cells. -
Flexible Workflow
Compatible with multiple cell types and assay conditions. -
Extensive Scientific Validation
Widely cited in peer-reviewed research publications.
The MCE Cell Counting Kit-8 (CCK-8) is a reliable, sensitive, and easy-to-use solution for cell viability and cytotoxicity analysis. Its streamlined workflow, high reproducibility, and compatibility with high-throughput screening make it an essential tool for modern cell biology, drug discovery, and biomedical research laboratories.
- Pack Size: 1 mL 5 mL 5 mL × 6 5 mL × 24