Liofilchem™ MTS™ Penicillin G P 0.016-256, MIC Test strips

The Liofilchem™ MTS™ Penicillin G P 0.016–256 MIC Test Strips are a reliable and precise tool for determining the Minimum Inhibitory Concentration (MIC) of Penicillin G against various bacterial pathogens. These test strips are widely used in clinical and research laboratories for antimicrobial susceptibility testing (AST).

Product Overview

• Drug Tested: Penicillin G (P)
• Concentration Range: 0.016–256 µg/mL
• Format: Plastic gradient MIC strips impregnated with Penicillin G
• Application: Quantitative determination of MIC values for Gram-positive and Gram-negative bacteria, specifically targeting penicillin-susceptible and penicillin-resistant strains.

Features

1. Gradient Technology: The strips feature a predefined gradient of Penicillin G concentrations, enabling precise MIC determination.
2. Ease of Use: Designed for direct application on inoculated agar plates.
3. Accuracy: Delivers reliable MIC results that align with international standards such as EUCAST and CLSI.
4. Interpretation: Results are directly readable at the point where the bacterial growth inhibition ellipse intersects the strip’s scale.

Testing Procedure

1. Preparation:
   • Use Mueller-Hinton Agar (MHA) or a specialized agar as recommended for the bacterial strain.
   • Inoculate the agar plate with a bacterial suspension adjusted to a 0.5 McFarland standard.
2. Application:
   • Place the MTS strip with the concentration gradient facing up on the inoculated plate.
   • Gently press to ensure proper adhesion.
3. Incubation:
   • Incubate the plate at 35°C ± 2°C under appropriate atmospheric conditions for 16–20 hours.
4. Interpretation:
   • Observe the point at which the elliptical zone of inhibition intersects the MIC scale on the strip.
   • Record the MIC value in µg/mL.

Quality Control

• Control Strains:
  • Staphylococcus aureus ATCC® 29213: Expected MIC range: 0.03–0.12 µg/mL.
  • Escherichia coli ATCC® 25922: Expected MIC range: ≤1 µg/mL.
• Performance Standards:
  • EUCAST and CLSI-approved MIC ranges are used for validation.
  • Regular quality control tests ensure precision and reproducibility.

Storage and Stability

• Storage Conditions:
  • Long-term: -20°C or below.
  • Short-term: 2–8°C (for up to 2 weeks).
  • Avoid exposure to moisture or light.
• Shelf Life: 24 months from the date of manufacture when stored appropriately.

Regulatory Data

1. Certifications:
   • CE-marked for in vitro diagnostic use (IVD) in the European Union.
   • Compliant with ISO 20776-1: Clinical laboratory testing standards for antimicrobial susceptibility testing.
2. Approvals:
   • Recommended for use in clinical microbiology laboratories worldwide.
   • CLSI and EUCAST standards ensure global acceptance.

Reference Data

1. Antimicrobial Susceptibility Testing Guidelines:
   • CLSI M100: Performance Standards for Antimicrobial Susceptibility Testing.
   • EUCAST Breakpoint Tables for Interpretation of MICs.
2. Bacterial Strain Testing:
   • Penicillin G is a first-line antibiotic for testing susceptibility in:
     • Staphylococcus spp.
     • Streptococcus spp.
     • Enterococcus spp.

Comparative Insights

• Liofilchem™ MTS™ vs. Broth Microdilution:
  • MTS strips are faster, require less reagent preparation, and offer comparable precision.
• Liofilchem™ MTS™ vs. Disk Diffusion:
  • Provides quantitative MIC values, whereas disk diffusion only offers categorical results (S/I/R).

Applications

• Clinical Microbiology:
  • Monitoring antimicrobial resistance patterns in hospitals.
• Research:
  • Evaluating new antibiotic formulations.
• Veterinary Medicine:
  • Testing susceptibility in animal pathogens.

Packaging

• 10, 30, or 100 test strips per box.
• Individually packaged in moisture-resistant pouches for optimal stability.

Advantages

1. Precise MIC determination.
2. Cost-effective alternative to broth microdilution methods.
3. Compliant with global regulatory standards.

The Liofilchem™ MTS™ Penicillin G P 0.016–256 MIC Test Strips provide reliable, reproducible, and rapid results, ensuring their essential role in combating antimicrobial resistance and guiding effective treatment decisions.