The Liofilchem™ MTS™ Meropenem/Meropenem+EDTA MIC Test Strips (MRP/MRD) are highly specialized tools for detecting carbapenem resistance mechanisms, specifically metallo-beta-lactamases (MBLs), in Gram-negative bacteria. These strips provide an accurate and efficient method for determining the Minimum Inhibitory Concentration (MIC) of Meropenem and evaluating the inhibitory effect of EDTA on metallo-beta-lactamase activity.
Key Features
- Dual Antibiotic Detection:
- Each strip contains a gradient for Meropenem (MRP) on one end and Meropenem + EDTA (MRD) on the other, enabling direct comparison of MIC values.
- Resistance Mechanism Detection:
- Differentiates metallo-beta-lactamase (MBL) producers by comparing MIC values of Meropenem alone versus Meropenem with EDTA.
- Wide MIC Range:
- Spanning 0.125–8 µg/mL for Meropenem and 0.032–2 µg/mL for Meropenem+EDTA, suitable for detecting low- and high-level resistance.
- Compliance with International Standards:
- Conforms to CLSI and EUCAST guidelines for antimicrobial susceptibility testing (AST).
- Precision Gradient Diffusion:
- Ensures accurate MIC determination with a continuous antibiotic gradient.
Applications
| Application | Description |
|---|
| Clinical Diagnostics | Identifies carbapenem resistance in Gram-negative pathogens, including MBL production. |
| Antimicrobial Resistance Studies | Tracks the emergence of carbapenem resistance in healthcare settings. |
| Pharmaceutical Research | Supports evaluation of Meropenem efficacy and MBL inhibitors like EDTA in new drug studies. |
Testing Procedure
- Preparation:
- Prepare a bacterial suspension to 0.5 McFarland standard.
- Spread the suspension evenly on Mueller-Hinton agar plates.
- Application:
- Place the MTS™ MRP/MRD strips onto the agar surface using sterile forceps.
- Incubation:
- Incubate plates at 35–37°C for 16–20 hours under aerobic conditions.
- Interpretation:
- Read the MIC values at the point where bacterial growth inhibition intersects the strip.
- Compare the MIC values for Meropenem (MRP) and Meropenem+EDTA (MRD):
- A reduction in MIC by ≥3 two-fold dilutions (8×) indicates MBL production.
Storage and Stability
| Condition | Temperature | Duration |
|---|
| Long-Term Storage | -20°C | Until expiration date |
| Short-Term Storage | 2–8°C | Up to 2 weeks |
| During Use | Room Temperature | Immediate use only |
Technical Specifications
| Feature | Details |
|---|
| Active Components | Meropenem and EDTA |
| MIC Range | Meropenem: 0.125–8 µg/mL; Meropenem+EDTA: 0.032–2 µg/mL |
| Test Method | Gradient diffusion |
| Compatible Media | Mueller-Hinton agar |
| Incubation Conditions | 35–37°C for 16–20 hours |
| Target Pathogens | Gram-negative bacteria, including MBL producers |
| Shelf Life | As per expiration date on packaging |
| Pack Sizes | 10 or 30 or 100 test strips per pack |
Quality Control Data
| Control Organism | Expected Results |
|---|
| Escherichia coli ATCC® 25922 | MRP MIC: 0.008–0.06 µg/mL; MRD MIC: Similar |
| Pseudomonas aeruginosa ATCC® 27853 | MRP MIC: 0.5–4 µg/mL; MRD MIC: No significant reduction |
| Klebsiella pneumoniae (MBL-positive strain) | MRP MIC: High (≥8 µg/mL); MRD MIC: Significant reduction |
| Staphylococcus aureus ATCC® 29213 | Not applicable (Gram-positive control) |
- Positive Controls: Confirm detection of MBL-producing strains.
- Negative Controls: Ensure no false-positive results in non-MBL producers.
Advantages
| Feature | Benefit |
|---|
| Dual Detection System | Identifies MBL production directly on primary testing. |
| High Sensitivity and Specificity | Accurate resistance detection minimizes false results. |
| Ease of Use | Simplified setup reduces laboratory errors and processing time. |
| Standards Compliance | Ensures reproducibility and reliability across laboratories. |
Comparison with Other MIC Test Strips
| Test Strip | MIC Range (µg/mL) | Recommended Use |
|---|
| Meropenem/Meropenem+EDTA (MRP/MRD) | MRP: 0.125–8; MRD: 0.032–2 | Detection of MBL resistance. |
| Meropenem 0.016–256 | 0.016–256 | General susceptibility testing. |
| Meropenem-vaborbactam 0.016/8–256/8 | 0.016/8–256/8 | Detection of beta-lactamase inhibition. |
Interpretation of Results
| MIC Value Comparison | Interpretation |
|---|
| MRP MIC = MRD MIC | Absence of MBL production. |
| MRP MIC ≥8× MRD MIC | Presence of MBL-producing bacteria. |
Conclusion
The Liofilchem™ MTS™ Meropenem/Meropenem+EDTA MRP/MRD MIC Test Strips are invaluable for detecting metallo-beta-lactamase activity in Gram-negative bacteria. Their dual detection system, high sensitivity, and adherence to international testing standards make them a reliable and efficient tool in combating antimicrobial resistance.
http://www.labmartgh.com/shop/product/liofilchem-tm-mts-tm-meropenem-meropenem-edta-mrp-mrd-0-125-8-0-032-2-mic-test-strips-28422
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Pack Size:
100/pk
10/pk
30/pk
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