Liofilchem™ G.N. Hajna Broth

Liofilchem™ G.N. Hajna Broth is a selective enrichment medium formulated for the cultivation and isolation of Gram-negative microorganisms, particularly Salmonella and Shigella, from clinical and non-clinical specimens. The medium contains a balanced mixture of nutrients, phosphate buffer, and selective agents that suppress Gram-positive flora and favor the growth of enteric pathogens.

The higher mannitol-to-glucose ratio and presence of sodium desoxycholate help maintain inhibitory conditions for normal fecal flora, while promoting early-phase growth of Shigella and Salmonella species.

Key Features

• Selective Enrichment: Supports recovery of Gram-negative enteric pathogens while inhibiting Gram-positive bacteria.
• Optimized Nutrient Balance: Contains tryptose and phosphate buffer to promote bacterial growth and maintain pH stability.
• Enhanced Detection of Pathogens: Formulated to encourage Shigella and Salmonella proliferation in the early incubation phase.
• Convenient Format Options: Available as dehydrated powder or ready-to-use tubes for flexible laboratory workflows.
• Reliable Quality Control: Tested with key reference strains to ensure consistent performance and selectivity.

Typical Formula (per litre)

Preparation

1. Suspend 39.0 g of powder in 1 L of distilled or deionized water.
2. Heat to boiling and shake until completely dissolved.
3. Distribute into tubes.
4. Sterilize by autoclaving at 121 °C for 15 minutes.

Technique

1. Inoculate 1 g of feces or 1 mL of liquid stool into the broth, or insert swab specimens directly.
2. Emulsify the specimen thoroughly.
3. Incubate aerobically at 36 ± 1 °C for 6–8 hours.
4. Transfer 1–2 drops of incubated broth onto selective plate media.
5. Incubate plates aerobically at 36 ± 1 °C for 18–24 hours, then examine for pathogens.

Quality Control

Prepared medium: Light amber, slightly opalescent, without precipitate.

Incubation conditions: 36 ± 1 °C for 24 hours.

Storage

• Dehydrated medium: Store at 10–30 °C in a dry environment, tightly closed.
• Prepared tubes: Store at 2–8 °C.
• Use before expiry date or if no signs of deterioration or contamination are present.

Available Formats

Liofilchem™ G.N. Hajna Broth offers reliable and selective enrichment for Gram-negative bacteria, particularly Salmonella and Shigella, from complex specimens. With its carefully optimized composition and consistent quality, it is an excellent choice for clinical, food, and environmental microbiology laboratories requiring dependable enteric pathogen recovery.