Liofilchem® Agar Powder

Liofilchem® Agar is a high-purity, bacteriological-grade solidifying agent used for the preparation of microbiological culture media. Derived from red algae (Rhodophyceae), this agar has been carefully refined to remove pigments, salts, and impurities, resulting in a clean, colorless medium base ideal for general and selective culture media formulations.

The product provides consistent gel strength, excellent clarity, and reliable pH stability, making it suitable for a wide range of bacteriological and biochemical applications. Liofilchem® Agar ensures uniform solidification of media and supports optimal growth conditions for both aerobic and anaerobic microorganisms.

Key Features

• High Purity: Free from extraneous matter and pigments for reproducible media results.
• Excellent Solidifying Agent: Forms stable gels at concentrations of 1–2% for general culture media.
• Versatile Applications: Suitable for solid media, motility studies, and anaerobic culture support.
• Controlled Gelation and Melting Points: Gels at 35°C and melts at 88°C, ensuring media integrity at incubation temperatures.
• Consistent Gel Strength: Provides firm and uniform media surfaces for microbial isolation.
• Complies with USP and bacteriological standards.
• Non-hazardous and easy to handle.

Applications

• Preparation of bacteriological and mycological culture media
• Solidification of nutrient, selective, and differential agar formulations
• Motility testing of microorganisms (at 0.5% w/v)
• Cultivation of anaerobic and microaerophilic organisms (at 0.1% w/v)
• Research and quality control laboratories requiring high-grade agar solids

Physico-Chemical Characteristics

Preparation and Use

• Use at 1–2% concentration (10–20 g/L) for standard solid media preparation.
• For motility or semi-solid media, use 0.05–0.5% agar concentration.
• Dissolve in distilled or deionized water by heating, and sterilize by autoclaving (121°C, 15 min).
• Once solidified (~35°C), agar remains stable at typical incubation temperatures (37°C).
• Ensures uniform diffusion and proper oxygen gradients, facilitating growth of fastidious and anaerobic organisms.

Storage and Stability

• Store at 10–30°C, protected from moisture.
• Keep container tightly closed when not in use.
• Avoid extreme temperature fluctuations.
• Use before the expiry date indicated on the label or if deterioration is not evident (e.g., caking, discoloration).

Safety and Handling

• This product is not classified as hazardous under current EU and international legislation.
• Refer to the Safety Data Sheet (SDS) for handling recommendations.
• Dispose of unused or waste material in accordance with local and national regulations.

Quality Control

Ordering Information

Liofilchem® Agar provides reliable performance in bacteriological media preparation, ensuring reproducible results in both research and routine diagnostics. Its controlled gelation characteristics, low impurities, and superior clarity make it an ideal choice for laboratories preparing custom media or performing microbial growth studies.