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Invitrogen™ PureLink™ Genomic DNA Mini Kit
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Sample Type: Bacteria, Blood, Cells, Tissue
Specifications:
| Application | Genomic Purification | ||
| Product Type | Sample Prep | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ PureLink™ Genomic DNA Mini Kit provides a robust and versatile solution for high-yield, high-purity genomic DNA (gDNA) extraction from a wide range of biological sample types, including blood, tissues, cultured cells, bacteria, yeast, buccal swabs, and more. This silica spin column-based system is optimized for easy and efficient genomic DNA purification, ensuring contaminant-free DNA suitable for various downstream molecular biology applications.
Key Features
- Versatile Sample Compatibility:
- Designed for DNA extraction from various sources, including:
- Whole blood (up to 1 mL)
- Tissues (up to 25 mg)
- Cultured cells (up to 10⁶ cells)
- Bacteria, yeast, buccal swabs, dried blood spots, FFPE tissue, and saliva.
- Designed for DNA extraction from various sources, including:
- High Yields and Purity:
- Delivers ultraclean gDNA with minimal contamination, yielding DNA with A260/A280 ratios close to 1.9, ensuring excellent purity.
- Optimized Workflow:
- Specialized lysis buffers and optimized spin columns ensure consistent and efficient DNA recovery.
- Scalable Formats:
- Available in sizes for 10, 50, and 250 preps to meet varying throughput needs.
- Quick Processing:
- Complete purification in as little as 15 minutes after cell lysis.
Specifications
General Specifications (All Formats)
| Feature | Details |
|---|---|
| Technology | Silica Spin Column |
| Scale | Mini |
| Applications | Genotyping, qPCR, Southern blotting, sequencing, PCR |
| High-Throughput Compatibility | Manual use only; not compatible with automated systems |
| Elution Volume | 25–200 μL |
| Final Product | Genomic DNA (gDNA) |
| Shipping Condition | Room Temperature |
| Storage Conditions | Room temperature; Proteinase K and RNase A can be stored at 4°C for long-term use |
Yield and Starting Material
| Sample Type | Starting Material Amount | Yield |
|---|---|---|
| Whole Blood | ≤1 mL | ≤40 μg |
| Tissue | ≤25 mg | ≤40 μg |
| Cells (Cultured) | ≤10⁶ cells | ≤40 μg |
| Bacteria | ≤10⁹ cells | ≤40 μg |
| Yeast | ≤10⁷ cells | ≤40 μg |
| FFPE | ≤8 sections (5–15 μm thick) | ≤40 μg |
| Saliva (Oragene Preserved) | ≤4 mL | ≤40 μg |
| Dried Blood Spots | ≤5 punches | ≤40 μg |
Kit Contents for Invitrogen™ PureLink™ Genomic DNA Mini Kit
| Component | 10 Prep Kit | 50 Prep Kit | 250 Prep Kit |
|---|---|---|---|
| Genomic Lysis/Binding Buffer | 2.0 mL | 10 mL | 50 mL |
| Genomic Digestion Buffer | 1.8 mL | 9 mL | 45 mL |
| Genomic Wash Buffer 1 | 2.0 mL | 10 mL | 50 mL |
| Genomic Wash Buffer 2 | 1.5 mL | 7.5 mL | 37.5 mL |
| Genomic Elution Buffer | 2.0 mL | 10 mL | 50 mL |
| RNase A (20 mg/mL) | 0.4 mL | 1 mL | 5 mL |
| Proteinase K (20 mg/mL) | 0.2 mL | 1 mL | 5 mL |
| Spin Columns with Collection Tubes | 10 columns | 50 columns | 5 x 50 columns |
| Collection Tubes (2.0 mL) | 20 tubes | 100 tubes | 5 x 100 tubes |
Comparison with Competitor Kits
| Feature | Invitrogen PureLink Genomic DNA Mini Kit | Qiagen DNeasy Blood & Tissue Kit | Zymo Quick-DNA Universal Kit |
|---|---|---|---|
| Sample Types | Blood, tissue, cells, bacteria, yeast, saliva, swabs, FFPE | Blood, tissue, cells, bacteria, yeast | Blood, tissue, cells, bacteria, FFPE |
| Yield | ≤40 μg DNA | ≤30 μg DNA | ≤25 μg DNA |
| Processing Time | ~15 minutes after lysis | ~20 minutes | ~15 minutes |
| High-Throughput Compatibility | No | No | Yes |
| Elution Volume | 25–200 μL | 50–200 μL | 10–200 μL |
| RNase A Included | Yes | Yes | Yes |
| Storage | Room temperature | Room temperature | Room temperature |
Advantages
| Feature | Benefit |
|---|---|
| Versatility | Single kit compatible with multiple sample types. |
| Enhanced Purity | High-quality DNA with minimal contamination (A260/A280 ≈ 1.9). |
| Efficient Recovery | Yields up to 40 μg DNA with consistent reproducibility. |
| Scalable Formats | Available in 10, 50, and 250 prep sizes to suit diverse workflows. |
| Streamlined Workflow | Quick processing with easy-to-follow protocols. |
Applications
- Genotyping:
- Isolate DNA for accurate identification of genetic variations.
- PCR and Real-Time qPCR:
- Obtain high-quality DNA for reliable amplification.
- Sequencing:
- Use for Sanger or NGS applications.
- Blotting Techniques:
- Ideal for Southern blotting and hybridization assays.
Suggested Pairing
- Downstream Applications:
- Pair with Invitrogen™ Platinum™ Taq DNA Polymerase for PCR amplification.
- Quantification:
- Use Thermo Scientific™ NanoDrop™ Spectrophotometers for nucleic acid quantification.
The Invitrogen™ PureLink™ Genomic DNA Mini Kit is a reliable and versatile solution for high-quality genomic DNA isolation across various sample types, ensuring exceptional performance in downstream molecular biology workflows.
- Reactions: 50 rxn 250 rxn 10 rxn