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Invitrogen™ PureLink™ Fast Low-Endotoxin Midi Plasmid Purification Kit
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Specifications:
| Application | DNA Extraction and Purification | ||
| Storage Temperature | Room Temperature, 2-8°C | ||
| Product Type | Sample Prep | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ PureLink™ Fast Low-Endotoxin Midi Plasmid Purification Kit is a next-generation solution for rapid and efficient purification of transfection-grade plasmid DNA from E. coli. Leveraging advanced silica membrane columns and a precipitation-free protocol, the kit delivers high-quality DNA in less than 30 minutes.
Capable of purifying up to 0.4 mg plasmid DNA from 25–100 mL bacterial cultures, this kit ensures low endotoxin content (<1 EU/µg), making it ideal for standard transfections, cloning, sequencing, and other molecular biology workflows.
Key Features
- Ultra-Fast Protocol – Transfection-grade DNA purified in ~30 minutes.
- High Yield – Isolate up to 0.4 mg plasmid DNA from midi-scale cultures.
- Low Endotoxin Levels – Delivers DNA with <1 EU/µg endotoxins.
- Precipitation-Free – No ethanol precipitation required, ensuring reproducibility.
- Colored Buffers – Visual confirmation of bacterial lysis and neutralization prevents workflow errors.
- Vacuum-Based – Designed for use with a vacuum manifold (e.g., Thermo Scientific FastVac™) for maximum efficiency.
Applications
- Preparation of plasmid DNA for mammalian transfection.
- Cloning and ligation workflows.
- DNA sequencing (manual and automated).
- PCR amplification and in vitro transcription.
- Restriction digestion and labeling applications.
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | Plasmid Purification Kit (Midi) |
| Catalog Numbers | A35892 (25 preps), A36227 (50 preps) |
| Isolation Technology | Advanced silica membrane columns |
| Sample Volume | 25–100 mL E. coli culture |
| Yield | Up to 0.4 mg plasmid DNA |
| DNA Quality | <1 EU/µg endotoxin; transfection-grade |
| Test Time | 30 minutes |
| Format | Vacuum-compatible spin columns |
| Applications | Sequencing, cloning, PCR, transfection |
| Shipping Condition | Room temperature |
| Storage Condition | Buffers at RT; resuspension buffer at 4 °C |
| Unit Size | Each kit (25 or 50 preps) |
Kit Contents
- Resuspension Buffer (store at 4 °C)
- Lysis Buffer
- Precipitation Buffer
- Binding Buffer
- Wash Buffer 1
- Wash Buffer 2 (concentrate)
- Elution Buffer
- Column assemblies
- Syringe filters
- Syringe plungers
- Collection tubes
Unique Advantages
- 30-minute workflow significantly faster than traditional plasmid purification.
- Low-endotoxin DNA suitable for sensitive cell-based applications.
- Error-proof processing with colored buffers for visual verification.
- Vacuum-driven purification streamlines plasmid DNA isolation, improving throughput and reproducibility.
The Invitrogen™ PureLink™ Fast Low-Endotoxin Midi Plasmid Purification Kit delivers fast, high-yield, and low-endotoxin plasmid DNA from midi-scale cultures. With its advanced silica membrane technology, precipitation-free workflow, and transfection-grade results, it is an ideal solution for laboratories conducting cloning, sequencing, PCR, and mammalian transfection experiments.
- Pack Size: For 50 Preps For 25 preps