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Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Elisa Kit | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Human TGF beta-1 ELISA Kit is a solid-phase sandwich ELISA designed for the quantitative measurement of human Transforming Growth Factor beta-1 (TGFβ1 / TGFB1) in serum, plasma, buffered solutions, and cell culture supernatants. The kit recognises both natural and recombinant human TGF beta-1, making it suitable for cytokine research, inflammation studies, fibrosis research, oncology research, and cell signalling investigations.
The assay uses a pre-coated 96-well microplate with a target-specific capture antibody. Samples, standards, or controls bind to the immobilised antibody, followed by a biotinylated detection antibody and streptavidin-HRP. Signal development is measured using an absorbance microplate reader, with signal intensity proportional to the amount of TGF beta-1 present in the sample.
Key Features
- Quantitative ELISA kit for human TGF beta-1
- Recognises natural and recombinant human TGFβ1
- Solid-phase sandwich ELISA format
- Suitable for serum, plasma, and cell culture supernatant samples
- Low sample volume requirement: 20 µL
- Analytical sensitivity: 8.6 pg/mL
- Assay range: 31–2,000 pg/mL
- HRP-based colorimetric detection
- Supplied with pre-coated 96-well plate and required assay reagents
- Suitable for cytokine, fibrosis, oncology, and immune regulation research
- For Research Use Only
Applications
- Human TGF beta-1 quantification
- Cytokine expression analysis
- Inflammation and immune regulation studies
- Fibrosis research
- Oncology and tumour biology research
- Cell signalling studies
- Cell culture supernatant analysis
- Serum and plasma biomarker research
- Pharmaceutical and biotechnology research workflows
Product Specifications
| Parameter | Specification |
|---|---|
| Brand | Invitrogen™ |
| Product Name | Human TGF beta-1 ELISA Kit |
| Target / Protein Name | TGF beta-1 / TGFB1 |
| Species Tested | Human |
| Assay Format | Sandwich ELISA Kit |
| Detection System | HRP |
| Detector Antibody Conjugate | Biotin |
| Instrument Required | Absorbance microplate reader |
| Analytical Sensitivity | 8.6 pg/mL |
| Assay Range | 31–2,000 pg/mL |
| Sample Types | Serum, plasma, supernatant, buffered solution |
| Sample Volume | 20 µL |
| Hands-on Time | 1 hr 20 min |
| Time to Result | 4 hr 30 min |
| Homogeneous / No Wash | No |
| Interassay CV | 4.9% |
| Intraassay CV | 3.2% |
| Shipping Conditions | Wet ice |
| Storage | 2–8°C |
| Regulatory Status | Research Use Only |
Ordering Information
| Catalog Number | Product Size |
|---|---|
| BMS249-4 | 96 Tests |
| BMS249-4FIVE | 5 × 96 Tests |
Kit Contents
| Component |
|---|
| Pre-coated 96-well plate |
| Standard |
| Sample Diluent |
| Assay Buffer Concentrate |
| Biotinylated Detection Antibody |
| Streptavidin-HRP |
| Wash Buffer |
| Chromogen |
| Stop Solution |
| Adhesive Plate Covers |
Target Information
TGF beta-1 is a cytokine belonging to the transforming growth factor beta superfamily. It is involved in the regulation of cellular processes such as proliferation, differentiation, adhesion, migration, immune regulation, extracellular matrix remodelling, and tissue repair. TGF beta-1 is widely studied in cancer biology, fibrosis, inflammation, wound healing, and cell signalling research.
The Invitrogen™ Human TGF beta-1 ELISA Kit provides a sensitive and reproducible method for measuring human TGF beta-1 in biological samples. With its sandwich ELISA format, low sample volume requirement, defined assay range, and complete reagent set, it is a practical solution for laboratories investigating cytokine signalling, inflammation, fibrosis, tumour biology, and related biomedical research applications.
- Pack Size: 96 Tests Plate 5 x 96 Tests Plates