0
Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Elisa Kit | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Human Hemoglobin ELISA Kit is a sandwich ELISA designed for the quantitative measurement of human hemoglobin in serum, plasma, and cell culture supernatants. The assay exclusively recognizes both natural and recombinant human hemoglobin with high specificity.
This kit provides a sensitive and reproducible method for hemoglobin quantification, useful for research in hematology, anemia studies, thalassemia, sickle cell disease, and related applications.
Key Features
- High sensitivity: Analytical sensitivity of 1.5 ng/mL.
- Broad assay range: 1.5–400 ng/mL.
- Validated sample types: Serum, plasma, supernatant (50 µL per well).
- Reliable performance: Intra-assay CV <10%; Inter-assay CV <12%.
- Convenient format: Pre-coated 96-well plate and all essential reagents included.
- User-friendly protocol: Hands-on time ~1 hr 20 min; results in ~4 hr 45 min.
- Detection system: Biotinylated antibody + Streptavidin-HRP with TMB substrate.
- Instrument required: Standard absorbance microplate reader.
Kit Contents
- Pre-coated 96-well plate
- Hemoglobin standard
- Assay diluent concentrate
- Biotinylated detection antibody
- Streptavidin-HRP conjugate
- Wash buffer
- Chromogen substrate
- Stop solution
- Adhesive plate covers
Product Specifications
| Parameter | Specification |
|---|---|
| Format | Sandwich ELISA Kit |
| Detector antibody conjugate | Biotin |
| Label/Dye | HRP (horseradish peroxidase) |
| Sample Types | Serum, Plasma, Supernatant |
| Sample Volume | 50 µL |
| Assay Range | 1.5 – 400 ng/mL |
| Sensitivity | 1.5 ng/mL |
| Time to Result | ~4 hr 45 min |
| Hands-on Time | ~1 hr 20 min |
| Intra-Assay CV | <10% |
| Inter-Assay CV | <12% |
| Storage Conditions | 2–8°C (up to 6 months); –20°C for long-term |
| Shipping Conditions | Wet ice |
| Regulatory Status | For Research Use Only (RUO) |
Target Information
- Protein: Hemoglobin (Hb)
- Subunits: 2 alpha chains + 2 beta chains = HbA (~97% of adult hemoglobin)
- Other isoforms: HbA2 (α2δ2), HbF (α2γ2)
- Clinical Relevance: Studies on anemia, thalassemia, sickle cell disease, hemoglobinopathies.
- Gene Symbols: HBA1, HBA2, HBB
- Gene IDs (Human): 3039, 3043
- UniProt IDs: P69905 (α), P68871 (β)
Applications
- Quantification of hemoglobin levels in biological samples.
- Research on hematological disorders such as anemia, thalassemia, and sickle cell disease.
- Monitoring of hemoglobin expression in experimental studies.
- Quality control in hemoglobin-related research workflows.
The Invitrogen™ Human Hemoglobin ELISA Kit (EH237RB series) is a reliable, sensitive, and easy-to-use tool for accurate quantification of hemoglobin in serum, plasma, and culture supernatants. With its validated performance, broad dynamic range, and convenient format, it is an essential research kit for studies in hematology, genetics, and clinical research applications.
- Pack Size: 96 Tests Plate 5 x 96 Tests Plates 10 x 96 Tests Plates