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Invitrogen™ eBioscience™ Protein Transport Inhibitor Cocktail (500X)
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Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | -20°C | ||
| Product Type | Flow Cytometry Reagents | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ eBioscience™ Protein Transport Inhibitor Cocktail (500X) is a ready-to-use, highly concentrated inhibitor formulation containing Brefeldin A and Monensin, two well-established agents that block intracellular protein transport. The cocktail is designed to prevent secretion of newly synthesized proteins, enabling their accumulation within the endoplasmic reticulum (ER) and Golgi apparatus.
This product is widely used in flow cytometry–based intracellular cytokine staining (ICS), functional immune assays, and protein expression studies, where detection of intracellularly retained proteins is required.
Mechanism of Action
- Brefeldin A disrupts protein transport from the ER to the Golgi by inhibiting COPI-mediated vesicle formation.
- Monensin, an ionophore, interferes with Golgi function by altering intracellular ion gradients.
Together, these inhibitors block the secretory pathway, leading to intracellular accumulation of cytokines, chemokines, and other secreted proteins. This accumulation allows sensitive downstream detection using intracellular staining, flow cytometry, or Western blotting.
Key Features & Advantages
- Dual-inhibitor formulation (Brefeldin A + Monensin) for robust protein transport inhibition
- Highly concentrated (500X) – economical and flexible dosing
- Promotes intracellular accumulation of secreted proteins
- Optimized for intracellular cytokine detection
- Specifically formulated as a negative control for the Cell Stimulation Cocktail (plus protein transport inhibitors) (500X)
- Compatible with flow cytometry and Western blot analysis
- Trusted eBioscience™ quality and reproducibility
Applications
- Intracellular cytokine staining (ICS)
- Flow cytometric analysis of immune cell activation
- Functional T-cell and NK-cell assays
- Cell culture–based protein expression studies
- Western blot detection of intracellularly retained proteins
- Negative control in cell stimulation experiments
Reported Applications:
- Flow Cytometric Analysis
- Functional Assays
- Cell Culture
Product Formats & Ordering Information
| Catalog Number | Description | Quantity |
|---|---|---|
| 00-4980-93 | Protein Transport Inhibitor Cocktail (500X) | 100 µL |
| 00-4980-03 | Protein Transport Inhibitor Cocktail (500X) | 4 × 100 µL |
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Type | Protein Transport Inhibitor Cocktail |
| Active Components | Brefeldin A, Monensin |
| Concentration | 500X |
| Unit Size | Each |
| Application Method | Cell culture additive |
| Detection Methods | Flow cytometry, Western blot |
| Intended Use | Research use only |
Storage & Handling
- Store according to manufacturer’s instructions
- Thaw and mix gently before use
- Use aseptic technique when adding to cell cultures
The Invitrogen™ eBioscience™ Protein Transport Inhibitor Cocktail (500X) is an essential reagent for intracellular protein and cytokine analysis in immunology and cell biology research. By effectively blocking the secretory pathway, it enables accurate detection of intracellular proteins using flow cytometry and biochemical methods. Its dual-inhibitor composition, high concentration, and proven reliability make it a trusted choice for functional immune assays and cell signaling studies.
- Pack Size: 100 μL 4 x 100 μL