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Invitrogen™ CD45 Monoclonal Antibody (30-F11), FITC, eBioscience™
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Specifications:
| Application | Western Blot, immunocytochemistry, Flow Cytometry, Immunohistochemistry, Immunoprecipitation (IP) | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Primary Antibodies | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ CD45 Monoclonal Antibody (Clone 30-F11), FITC-conjugated is a highly specific antibody designed for the detection of CD45 (Leukocyte Common Antigen, LCA), a pan-leukocyte marker expressed on all nucleated hematopoietic cells. This antibody is directly conjugated to FITC (Fluorescein Isothiocyanate), enabling rapid and sensitive fluorescence-based detection in flow cytometry applications.
Optimized for immunophenotyping and immune cell analysis, this antibody is widely used in hematology, immunology, and oncology research for identifying and characterizing leukocyte populations.
Key Features
- Specific for CD45 (pan-leukocyte marker)
- Monoclonal antibody clone 30-F11
- Recognizes all isoforms of mouse CD45
- Conjugated with FITC fluorophore
- Excitation/Emission: 498 / 517 nm (blue laser compatible)
- Reactivity: Mouse (validated; cross-referenced in multiple species)
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Optimized for:
- Flow Cytometry: ≤0.5 µg/test
- Supplied as liquid, ready-to-use antibody
- Purified by affinity chromatography
- Sterile-filtered (0.2 µm) for performance consistency
Applications
This antibody is ideal for:
- Flow cytometric analysis of leukocytes
- Immunophenotyping of immune cell populations
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Identification of:
- T cells
- B cells
- Myeloid cells
- Bone marrow populations
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Research applications in:
- Immunology and hematology
- Cancer and tumor immunology
- Stem cell and progenitor cell studies
- Immune response profiling
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Invitrogen™ CD45 Monoclonal Antibody (30-F11), FITC |
| Catalog Numbers | 11-0451-81 / 82 / 85 |
| Type | Antibody |
| Class | Monoclonal |
| Clone | 30-F11 |
| Host / Isotype | Rat / IgG2b, κ |
| Species Reactivity | Mouse |
| Conjugate | FITC |
| Excitation / Emission | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Preservative | 0.09% sodium azide |
| Storage Conditions | 4°C, protect from light, do not freeze |
| Recommended Use | ≤0.5 µg/test (Flow Cytometry) |
| RRID | AB_465050 |
Target Information
CD45 (Leukocyte Common Antigen) is a receptor-type protein tyrosine phosphatase expressed on all nucleated cells of hematopoietic origin, excluding mature erythrocytes and platelets. It plays a central role in immune cell signaling, particularly in the activation of T and B lymphocytes through regulation of Src-family kinases.
CD45 exists in multiple isoforms generated by alternative splicing, with expression patterns varying across immune cell subsets and activation states. It is a critical marker for distinguishing hematopoietic cells from non-hematopoietic cells and is widely used in immune profiling and flow cytometry panels.
Advantages
- Reliable pan-leukocyte marker for immune cell identification
- Direct FITC conjugation simplifies workflow (no secondary antibody required)
- High specificity and reproducibility
- Ideal for flow cytometry and multiparametric analysis
- Widely cited and validated in immunological studies
The Invitrogen™ CD45 Monoclonal Antibody (30-F11), FITC is a robust and essential reagent for immune cell analysis and flow cytometry applications. Its strong specificity for leukocytes, ease of use, and compatibility with fluorescence-based platforms make it a cornerstone tool for immunology, hematology, and cancer research laboratories.
- Pack Size: 50 µg 500 µg 100 µg