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Invitrogen™ CD44 Monoclonal Antibody (IM7), eBioscience™
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Specifications:
| Application | Western Blot, immunocytochemistry, ELISA-Based Assays, Flow Cytometry, Immunohistochemistry, Immunoprecipitation (IP), Functional Assay (FN) | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Primary Antibodies | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ CD44 Monoclonal Antibody (Clone IM7) is a highly specific antibody targeting CD44, a multifunctional cell surface glycoprotein involved in cell adhesion, migration, and signaling. Recognizing all isoforms of mouse CD44 (Pgp-1), this antibody is widely used in immunology, oncology, and stem cell research.
CD44 is expressed on both hematopoietic and non-hematopoietic cells, including lymphocytes, epithelial cells, and tumor cells, and plays a critical role in cell–cell interactions and extracellular matrix binding, particularly through its interaction with hyaluronic acid (HA).
Key Features
- Specific for CD44 (Pgp-1) cell surface antigen
- Monoclonal antibody clone IM7
- Reactivity: Human and Mouse
- Recognizes all CD44 isoforms
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Validated for multiple applications:
- Flow Cytometry: ≤0.125 µg/test
- Western Blot: 1 µg/mL
- Immunocytochemistry (ICC/IF): 1:500
- Immunohistochemistry (IHC)
- Immunoprecipitation (IP)
- High purity (>90%) and low aggregation (<10%)
- Supplied as unconjugated antibody for flexible assay design
- Purified by affinity chromatography
Applications
This antibody is ideal for:
- Flow cytometry analysis of immune and stem cell populations
- Immunohistochemistry and tissue staining
- Western blot detection of CD44 expression
- Immunoprecipitation and functional assays
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Research in:
- Cell adhesion and migration
- Cancer metastasis and tumor progression
- Stem cell and cancer stem cell biology
- Immune cell activation and homing
- Extracellular matrix interactions
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Invitrogen™ CD44 Monoclonal Antibody (IM7) |
| Catalog Numbers | 14-0441-80 / 82 / 85 / 86 |
| Type | Antibody |
| Class | Monoclonal |
| Clone | IM7 |
| Host / Isotype | Rat / IgG2b, κ |
| Species Reactivity | Human, Mouse |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Preservative | 0.09% sodium azide |
| Storage Conditions | 4°C |
| RRID | AB_467246 |
Target Information
CD44 is a type I transmembrane glycoprotein (~100 kDa) that functions primarily as a receptor for hyaluronic acid, facilitating cell adhesion, migration, and signaling. It is widely expressed across various cell types, including immune cells, epithelial cells, and tumor cells.
CD44 plays a key role in:
- Lymphocyte activation and homing
- Cell–matrix interactions
- Tumor invasion and metastasis
Alternative splicing of the CD44 gene generates multiple isoforms, contributing to its functional diversity in physiological and pathological processes. Elevated CD44 expression is often associated with cancer progression and metastatic potential, making it an important biomarker in oncology research.
Advantages
- Broad application versatility (Flow, WB, IHC, ICC, IP)
- Recognizes multiple CD44 isoforms
- High specificity and reproducibility
- Suitable for immune profiling and cancer research
- Flexible unconjugated format for customized workflows
The Invitrogen™ CD44 Monoclonal Antibody (IM7) is a versatile and reliable reagent for studying cell adhesion, immune responses, and tumor biology. Its strong validation across multiple applications and ability to detect all CD44 isoforms make it an essential tool for immunology, oncology, and stem cell research laboratories.
- Pack Size: 1 mg 50 µg 500 µg 100 µg