Invitrogen™ CD32 Monoclonal Antibody (6C4 (CD32)), APC, eBioscience™

The Invitrogen™ CD32 Monoclonal Antibody (6C4), APC, eBioscience™ is a high-quality antibody designed for flow cytometry applications, specifically targeting human CD32 (Fc gammaRII). This APC-conjugated monoclonal antibody is optimized for detecting CD32 isoforms Fc gammaRIIA and Fc gammaRIIB, which are expressed on B cells, monocytes, granulocytes, macrophages, and platelets.

CD32 plays a crucial role in immune complex clearance, inflammation, and autoimmune disease mechanisms. This antibody is validated for high-specificity binding and is ideal for immunophenotyping, immune cell characterization, and functional studies in research.

Key Features & Benefits

1. High-Specificity Detection of CD32 (Fc gammaRII)

• Recognizes CD32A and CD32B isoforms, ensuring comprehensive CD32 profiling.
• Suitable for studying immune responses, Fc receptor interactions, and phagocytosis pathways.
• Tested and validated for flow cytometry, providing clear and reproducible staining results.

2. APC Conjugation for Enhanced Fluorescence

• Conjugated to Allophycocyanin (APC) for strong red fluorescence (Excitation: 651 nm, Emission: 660 nm).
• Compatible with multi-color flow cytometry panels using a red laser (633-647 nm).
• Ensures minimal spectral overlap, enhancing data accuracy in complex immunophenotyping experiments.

3. Ready-to-Use for Flow Cytometry Applications

• Pre-titrated for optimal staining at 5 µL (0.5 µg)/test.
• Filtered through a 0.2 µm membrane for maximum purity and batch-to-batch consistency.
• Supplied in PBS buffer (pH 7.2) with 0.2% BSA and 0.09% sodium azide for long-term stability.

4. Highly Reproducible & Published in Research

• Validated in peer-reviewed studies, demonstrating reliable performance.
• Used in immunology, inflammation, autoimmune disease, and infectious disease research.

Product Specifications

Available Variants & Ordering Information

Other Formats Available:

Use Cases & Applications

1. Immunophenotyping & Flow Cytometry

• Characterization of immune cell populations expressing CD32.
• Multi-color flow cytometry panels for studying Fc receptor interactions.

2. Inflammation & Autoimmune Disease Research

• Investigating CD32’s role in immune complex recognition and clearance.
• Studying Fc receptor involvement in autoimmune conditions.

3. Infectious Disease & Immunology Studies

• Assessing monocyte and granulocyte responses to infections.
• Exploring natural killer (NK) cell interactions with opsonized antigens.

Published Research & References

• Cytometry Part A (2018): Application of CD32 antibody in antibody-dependent cell-mediated cytotoxicity (ADCC) assays.
• Mediators of Inflammation (2017): Role of CD32 in autoimmune disease research.
• Journal of Neurovirology (2017): CD32 detection in microglial cells related to HIV research.

The Invitrogen™ CD32 Monoclonal Antibody (6C4), APC, eBioscience™ is a high-specificity reagent for flow cytometry-based immune cell characterization. Featuring APC conjugation for bright fluorescence, validated performance, and minimal spectral overlap, this antibody is an ideal choice for immunophenotyping, Fc receptor studies, and autoimmune research.