Human Yellow Fever Virus (YFV) ELISA Kit, Qualitative

The Human Yellow Fever Virus (YFV) IgG / IgM ELISA Kits are enzyme-linked immunosorbent assays designed for the qualitative detection of anti-YFV antibodies in human serum, plasma, culture media, and other biological fluids. These kits provide a reliable method for assessing immune response to Yellow Fever Virus infection in research and surveillance settings.

Based on a highly sensitive HRP-TMB colorimetric ELISA principle, the assay enables accurate detection of antibodies by measuring absorbance at 450 nm, allowing clear differentiation between positive and negative samples.

Key Features

• Detection of YFV-specific IgG and IgM antibodies
• Qualitative results (positive/negative interpretation)
• High sensitivity using HRP-conjugate and TMB substrate system
• Available in 48- and 96-well formats
• Suitable for multiple sample types:
  • Serum
  • Plasma
  • Cell culture supernatants
  • Biological fluids
• Includes positive and negative controls for validation
• Pre-coated antigen microplate for consistent performance
• Optimized for standard ELISA workflows

Applications

This kit is ideal for:

• Research detection of Yellow Fever Virus antibodies
• Serological studies and immune response analysis
• Epidemiological surveillance and outbreak research
• Differentiation of:
  • Recent infection (IgM)
  • Past exposure or immunity (IgG)
• Arbovirus research including:
  • Yellow Fever
  • Dengue
  • Zika

Principle of the Assay

The assay is based on a sandwich/indirect ELISA principle:

1. Microplate wells are pre-coated with YFV-specific antigen
2. Antibodies in the sample bind to the immobilized antigen
3. HRP-labelled conjugate forms an antigen–antibody complex
4. Addition of TMB substrate produces a blue color
5. Reaction is stopped, yielding a yellow color proportional to antibody concentration
6. Optical density is measured at 450 nm and compared to a cut-off value

Kit Components

• Antigen-coated microplate
• HRP-conjugate reagent
• Sample diluent
• Positive and negative controls
• Chromogen Solution A & B (TMB)
• Stop solution
• Wash buffer concentrate
• Adhesive plate seals

Technical Specifications

Advantages

• Reliable qualitative antibody detection
• Compatible with standard ELISA plate readers
• Flexible sample compatibility
• Complete kit with all required reagents
• Cost-effective solution for research and surveillance studies
• Easy-to-follow protocol for reproducible results

Important Notes

• For Research Use Only (RUO) – not for diagnostic or therapeutic use
• Avoid samples containing sodium azide, as it inhibits HRP activity
• Ensure proper washing steps to maintain assay precision
• Protect substrate reagents from light exposure

The Human Yellow Fever Virus (YFV) IgG / IgM ELISA Kits provide a reliable and efficient solution for the qualitative detection of YFV antibodies in research settings. With robust performance, flexible sample compatibility, and straightforward workflow, these kits are well-suited for arbovirus research, immunological studies, and epidemiological monitoring.