Human Interferon γ (IFN-γ) ELISA Kit

The Human Interferon γ (IFN-γ) ELISA Kit is designed for the quantitative measurement of IFN-γ concentrations in serum, plasma, cell culture supernatants, and other biological fluids.

Interferon-γ is a critical cytokine secreted primarily by activated T cells and NK cells, playing a central role in immune regulation, inflammation, and antiviral responses.

This kit employs a high-sensitivity sandwich ELISA method, pre-coated with a monoclonal antibody specific for human IFN-γ, ensuring precise and reproducible quantification suitable for immunology and cell biology research.

Key Features

• High Sensitivity: Detection limit of 9.4 pg/mL with an assay range of 15.6–1,000 pg/mL.
• Precision Performance: Intra-assay CV < 10%, inter-assay CV < 12%.
• Comprehensive Kit: Includes standards, buffers, HRP conjugate, and substrate reagents for 96 determinations.
• Rapid Assay Time: Total time approximately 3 hours.
• Reliable Quantification: Linear standard curve with reproducible optical density readings at 450 nm.
• Validated Protocol: Based on the sandwich ELISA principle with HRP-TMB colorimetric detection.
• Quality Controlled: Manufactured under strict production and testing guidelines for consistent lot-to-lot reproducibility.

Principle of the Assay

This assay is based on a sandwich ELISA technique:

1. The 96-well microplate is pre-coated with anti-human IFN-γ antibody.
2. Samples and standards are added, allowing IFN-γ to bind the immobilized antibody.
3. A HRP-conjugated detection antibody binds to the captured IFN-γ.
4. Following washing, TMB substrate is added for color development.
5. The reaction is terminated, and absorbance is measured at 450 nm, proportional to IFN-γ concentration.
6. Concentrations are determined via comparison with a standard curve.

Specifications

Kit Components (96 Tests)

Applications

• Quantification of human IFN-γ in serum or plasma
• Cytokine profiling in immunological and inflammatory response research
• Cell culture supernatant analysis for cytokine secretion studies
• Immunotherapy and vaccine development research
• Autoimmune and infectious disease studies related to IFN-γ signaling pathways

Safety and Regulatory Notes

• For Research Use Only (RUO): Not for therapeutic, diagnostic, or clinical applications.
• Store all reagents at 2–8 °C and avoid repeated freeze–thaw cycles.
• Handle all human-derived materials as potentially infectious.
• Reagents from different lot numbers should not be mixed.

The Human Interferon γ (IFN-γ) ELISA Kit delivers sensitive, accurate, and reproducible detection of IFN-γ in a variety of biological samples.

With its robust performance, simple protocol, and high-quality reagents, it is an indispensable tool for immunological, biomedical, and cytokine research laboratories investigating cellular immune responses and inflammatory mechanisms.