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Human Immunoglobulin E (IgE) ELISA Kit
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Wavelength: 450nm
Assay range: 6ng/ml-400ng/ml
Sensitivity:1.5ng/ml
Standard: 540ng/ml
Sample Volume: 10ul
Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Elisa Kit | Forms | Kit with Various components |
| Product Grade | Molecular Biology | ||
The Human Immunoglobulin E (IgE) ELISA Kit is a high-sensitivity sandwich ELISA designed for the quantitative measurement of IgE in human biological fluids. It is suitable for use with serum, plasma, urine, cerebrospinal fluid, pleural/peritoneal fluid, culture supernatants, and tissue homogenates.
As IgE is a key mediator of allergic responses, parasitic infections, and certain autoimmune disorders, this assay is an essential tool in immunology, allergy, oncology, and clinical research.
Key Features
- High Sensitivity – Detects as low as 1.5 ng/mL.
- Broad Assay Range – 6 – 400 ng/mL.
- Reliable Reproducibility – Intra-assay CV < 10%; Inter-assay CV < 12%.
- Flexible Formats – Available in both 48T and 96T pack sizes.
- Wide Sample Compatibility – Validated for multiple biological fluids.
- Comprehensive Kit – Pre-coated plate, standards, conjugate, diluents, substrates, buffers, and stop solution included.
- Research Use Only – For laboratory research applications; not for therapeutic or diagnostic use.
Kit Contents
- Pre-coated MicroELISA Strip Plate (48 or 96 wells depending on kit size)
- Standards (540 ng/mL) & Standard Diluent
- HRP-Conjugate Reagent
- Sample Diluent
- Chromogen Solutions A & B
- Stop Solution
- Wash Buffer (Concentrate)
- Closure Plate Membrane
- Sealed Storage Bag
- User Manual
(Reagent volumes are scaled according to 48T or 96T format.)
Technical Specifications
| Parameter | Details |
|---|---|
| Catalogue Numbers | SL0913Hu-48 (48T), SL0913Hu (96T) |
| Assay Type | Sandwich ELISA |
| Detection Range | 6 – 400 ng/mL |
| Sensitivity | 1.5 ng/mL |
| Precision | Intra-assay CV < 10%, Inter-assay CV < 12% |
| Sample Types | Serum, plasma, urine, CSF, pleural/peritoneal fluid, cell culture supernatant, tissue homogenates |
| Wavelength | 450 nm |
| Storage | 2–8 °C |
| Shelf Life | 6 months |
| Use | Research Use Only (RUO) |
Applications
- Allergy Research – Measuring IgE in hypersensitivity and allergic response models.
- Parasitology – Monitoring IgE levels in helminth and parasitic infections.
- Immunology & Autoimmunity – Investigating IgE-mediated pathways in chronic inflammation.
- Oncology & Hematology – Studying IgE’s role in rare immunoglobulin disorders and immune dysregulation.
Storage & Handling
- Store reagents at 2–8 °C.
- Shelf life: 6 months.
- Avoid repeated freeze–thaw cycles.
- Do not use with sodium azide (NaN₃), as it inhibits HRP activity.
Ordering Information
| Product | Catalogue Number | Pack Size | Storage | Validity |
|---|---|---|---|---|
| Human Immunoglobulin E (IgE) ELISA Kit | SL0913Hu-48 | 48 Tests | 2–8 °C | 6 months |
| Human Immunoglobulin E (IgE) ELISA Kit | SL0913Hu | 96 Tests | 2–8 °C | 6 months |
The Human Immunoglobulin E (IgE) ELISA Kit offers a sensitive, reproducible, and versatile solution for IgE quantification across diverse human samples. With broad sample compatibility, consistent performance, and availability in both 48-test and 96-test formats, this kit is an essential tool for researchers in allergy, parasitology, immunology, and autoimmune disease research.
- Pack Size: For 48 Tests For 96 Tests