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Specifications:
| Application | qPCR | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Master Mix | Forms | Liquid |
| Product Brand | Solis Biodyne | ||
| Product Grade | Molecular Biology | ||
The HOT FIREPol® EvaGreen® qPCR Supermix (5X) is a ready-to-use, hot-start master mix optimized for dye-based real-time quantitative PCR (qPCR).
Utilizing EvaGreen® dye and the SYBR®/FAM channel, this formulation provides high sensitivity, specificity, and reproducibility for DNA or cDNA quantification across a broad range of real-time PCR instruments.
Incorporating chemically modified HOT FIREPol® DNA Polymerase, this master mix minimizes nonspecific amplification and primer-dimer formation, while its blue visualization dye simplifies reaction setup without interfering with fluorescence detection.
Key Features
- High Sensitivity & Specificity: Optimized for detection of low DNA concentrations, including GC-rich targets.
- Hot-Start Technology: Chemically modified polymerase activated in 12 minutes prevents nonspecific amplification.
- EvaGreen® Dye System: Non-mutagenic, stable, and low-inhibition DNA-binding dye — superior to SYBR® Green I.
- Blue Visualization Dye: Facilitates accurate pipetting during assay setup.
- Carryover Prevention: Contains dUTP for contamination control with optional UNG treatment (UNG not included).
- Universal Compatibility: Works on most qPCR cyclers (low ROX and no ROX platforms).
- Reference Dye: Includes a ROX-based internal reference for normalization.
- GC Enhancer: Improves amplification of GC-rich templates.
- Research Use Only (RUO): Not for diagnostic or therapeutic procedures.
Specifications
| Parameter | Specification |
|---|---|
| Concentration | 5X |
| Hot-Start | Yes (activation in 12 minutes) |
| Detection Type | Dye-based (EvaGreen®) |
| Reference Dye | ROX (for normalization) |
| Polymerase | Chemically modified HOT FIREPol® DNA Polymerase |
| MgCl₂ Concentration (1X) | 2.5 mM |
| dNTP Composition | Includes dUTP for UNG treatment (UNG not included) |
| Form | Liquid master mix |
| Color Indicator | Blue pipetting dye |
| Storage Conditions | −20 °C; avoid freeze-thaw cycles |
| Shipping Conditions | Room temperature |
| Intended Use | Research Use Only (RUO) |
Applications
- Quantitative detection of DNA and cDNA targets
- Gene expression profiling and relative quantification
- Microbial and viral detection assays
- Genotyping and SNP analysis
- Viral load determination
- Standard curve and melt curve analysis
Compatibility
| Cycler Type | Compatibility |
|---|---|
| No ROX / Low ROX | ✅ Compatible |
| High ROX (e.g., Applied Biosystems StepOne™ / StepOnePlus™) | ❌ Not compatible |
| Other Common Platforms | Works with Bio-Rad, Qiagen, Roche, Agilent, Analytik Jena, and similar systems |
Mix Components
- HOT FIREPol® DNA Polymerase (chemically modified hot-start enzyme)
- EvaGreen® dye (non-toxic intercalating dye)
- 5X qPCR buffer with 12.5 mM MgCl₂
- dNTPs including dUTP (for UNG-mediated contamination control)
- ROX reference dye (low concentration for most instruments)
- GC enhancer for improved amplification of difficult templates
- Blue visualization dye (for accurate pipetting)
EvaGreen® Dye Advantages
- Low PCR inhibition and superior stability versus SYBR® Green I
- Non-cytotoxic and non-mutagenic — safer for laboratory use
- Compatible with all qPCR instruments that use FAM or SYBR® channels
- Excellent signal-to-noise ratio for highly specific detection
The HOT FIREPol® EvaGreen® qPCR Supermix (5X) is a high-performance, dye-based master mix designed for precise and reproducible real-time PCR quantification.
Featuring EvaGreen® dye, HOT FIREPol® DNA Polymerase, and blue visualization dye, it provides maximum sensitivity, ease of use, and robust amplification, even for GC-rich sequences.
Ideal for gene expression profiling, microbial detection, and viral quantification, this all-in-one qPCR reagent is compatible with most real-time cyclers and delivers Thermo Scientific–grade reliability.
- Pack Size: 1 x 1 mL, sufficient for 250 Rxns 5 x 1 mL, sufficient for 1250 Rxns 10 x 1 mL, sufficient for 2500 Rxns 1 x 20 mL, sufficient for 5000 Rxns 0.2 ml, 50 x 20 μl rxn, free sample (Terms & Conditions Applies) 2 x 20 mL, Sufficient for 10,000 Rxns 1 x 100 mL, Sufficient for 25,000 Rxns 2 x 100 mL, Sufficient for 50,000 Rxns