Gibco™ Human PDGF-AA Recombinant Protein, PeproTech®

Gibco™ Human PDGF-AA Recombinant Protein (PeproTech®) is a highly purified recombinant growth factor used in cell biology and biomedical research to study cell proliferation, differentiation, migration, and tissue repair mechanisms. PDGF-AA belongs to the platelet-derived growth factor family, a group of potent mitogenic cytokines involved in the regulation of connective tissue cell growth and development.

Recombinant Human PDGF-AA is produced in an E. coli expression system and supplied as a lyophilized, preservative-free protein with a purity of ≥98% by SDS-PAGE and HPLC analysis. The protein forms a disulfide-linked homodimer consisting of two A-chains with a total molecular weight of 28.5 kDa. This recombinant cytokine demonstrates strong biological activity by stimulating proliferation of Balb/c 3T3 fibroblast cells, with an expected ED50 of 1.0–3.0 ng/mL.

PDGF-AA is widely used in studies involving fibroblasts, smooth muscle cells, endothelial cells, and glial cells, where it functions as both a mitogenic and chemotactic factor. It also plays roles in collagen synthesis, immune modulation, neuronal survival, and wound healing pathways, making it a valuable tool in regenerative medicine, oncology, immunology, and neuroscience research.

Key Features

• High purity recombinant human growth factor (≥98%)
• Produced in E. coli expression system
• Supplied as lyophilized protein for long-term stability
• Low endotoxin level (<1 EU/µg) suitable for sensitive cell culture applications
• Highly potent bioactivity with ED50 of 1.0–3.0 ng/mL
• Stimulates proliferation of fibroblast and connective tissue cells
• Preservative-free formulation for flexible experimental use
• Extensively cited in scientific literature with numerous validated applications

Applications

• Cell proliferation and cell signaling studies
• Growth factor stimulation assays
• Fibroblast and smooth muscle cell culture
• Stem cell and regenerative medicine research
• Neuroscience and glial cell studies
• Oncology and tumor microenvironment research
• ELISA standards and Western blot controls
• Cytokine signaling pathway investigations

Available Sizes

Technical Specifications

Biological Function

Platelet-Derived Growth Factor (PDGF) is a key regulatory cytokine involved in cell growth, migration, and tissue repair. PDGF-AA is one of three major PDGF isoforms (AA, AB, and BB) and signals primarily through the PDGF receptor-α (PDGFR-α).

In biological systems, PDGF-AA:

• Stimulates proliferation of fibroblasts and smooth muscle cells
• Promotes chemotaxis of immune and connective tissue cells
• Regulates collagen synthesis and extracellular matrix remodeling
• Supports neuronal survival and glial cell proliferation
• Modulates immune signaling and inflammatory responses

These properties make PDGF-AA a critical reagent in cell signaling, wound healing, cancer biology, and regenerative medicine research.

Gibco™ Human PDGF-AA Recombinant Protein (PeproTech®) is a high-quality, bioactive cytokine reagent designed for advanced research applications in cell biology, regenerative medicine, neuroscience, and oncology. With exceptional purity, low endotoxin levels, and proven biological activity, it provides researchers with a reliable tool for investigating PDGF-mediated signaling pathways and cellular responses in vitro.