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Specifications:
| Application | Reverse Transcription | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Kit with Various components |
| Product Brand | Solis Biodyne | ||
| Product Grade | Molecular Biology | ||
The FIREScript® RT cDNA Synthesis Kit is a high-efficiency, flexible, and thermostable reverse transcription system designed for rapid and reliable first-strand cDNA synthesis from RNA templates.
At its core is the genetically modified FIREScript® Reverse Transcriptase (RT) — an MMLV (Moloney Murine Leukemia Virus)-based enzyme engineered for increased thermostability, enhanced sensitivity, and improved performance at elevated temperatures (37°C to 60°C).
The kit enables fast 15-minute cDNA synthesis, delivering high yield and specificity across a broad temperature range, with convenient reaction setup without the need for ice. It is ideal for RT-PCR and RT-qPCR applications in gene expression and RNA analysis workflows.
Key Features
- Fast and robust cDNA synthesis in as little as 15 minutes.
- High thermostability and superior performance at 37–60°C.
- Flexible priming options with Oligo(dT) and Random Primers included.
- Enhanced enzyme stability at room temperature (up to 1 month without activity loss).
- Complete 7-vial system with all reagents provided, including RNase inhibitor and nuclease-free water.
- Ready-to-use kit shipped and stored at room temperature; no ice required.
- Contains functional RNase H domain for increased RT-qPCR sensitivity.
Applications
- First-Strand cDNA Synthesis
- RT-PCR and RT-qPCR
- Gene Expression Profiling
- RNA Detection and Quantification
- Viral RNA Reverse Transcription Studies
Kit Components
| Component | Quantity / Concentration | Vial Size |
|---|---|---|
| FIREScript® Reverse Transcriptase | 10,000 U (200 U/µl) | 50 µl |
| RiboGrip® RNase Inhibitor (220 U/µl) | 2,000 U | 9 µl |
| 10× RT Reaction Buffer with DTT | – | 125 µl |
| dNTP Mix (20 mM each) | 2 µmol | 25 µl |
| Oligo (dT) Primer | 100 µM | 50 µl |
| Random Primers | 100 µM | 50 µl |
| Nuclease-Free Water | – | 1.25 ml |
Ordering Information
| Product | Catalog No. | Size | Packaging |
|---|---|---|---|
| FIREScript® RT cDNA Synthesis Kit (20 reactions) | 06-15-0000S | 20 rxn | Box |
| FIREScript® RT cDNA Synthesis Kit (50 reactions) | 06-15-00050 | 50 rxn | Box |
| FIREScript® RT cDNA Synthesis Kit (200 reactions) | 06-15-00200 | 200 rxn | Box |
Reagent Details
- Buffer Composition: 500 mM Tris-HCl (pH 8.3), 500 mM KCl, 30 mM MgCl₂, 100 mM DTT.
- Primers Included: Oligo(dT) and Random Primers for flexible priming strategies.
- Enzyme Type: Modified MMLV Reverse Transcriptase with RNase H domain.
- Compatible Assays: End-point RT-PCR, Quantitative RT-qPCR.
Storage & Shipping
- Routine Storage: –20°C.
- Shipping: At room temperature (stable up to 1 month).
- Stability: Maintains full activity after temporary room temperature storage and shipment.
Licensing and Use
Some applications of this product may require additional licenses not included with the purchase.
For research use only. Not for diagnostic or therapeutic applications.
The FIREScript® RT cDNA Synthesis Kit provides a flexible, high-yield reverse transcription system optimized for speed, thermostability, and sensitivity. It is the ideal choice for researchers performing RT-PCR and RT-qPCR workflows requiring consistent, high-quality cDNA synthesis. With its enhanced enzyme performance and room-temperature stability, FIREScript® offers a reliable, convenient, and efficient solution for modern molecular biology applications.
- Pack Size: 50 reactions 200 Reactions