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Invitrogen™ Fluorescein Di-β-D-Glucopyranoside (FDGlu), 5mg
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Specifications:
| Application | Fluorescence-Based Assay | ||
| Storage Temperature | -5 to -30°C | ||
| Product Type | Molecular Biology Reagent | Forms | Solid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | Formula | C₃₂H₃₂O₁₅ |
Highly sensitive, cell-permeant fluorogenic β-glucosidase substrate for biochemical and live-cell assays
Invitrogen™ FDGlu is a nonfluorescent, cell-permeant fluorogenic substrate specifically designed for β-glucosidase detection in biochemical assays and live-cell applications. Upon sequential enzymatic hydrolysis, FDGlu is first converted to fluorescein monoglucoside and then to highly fluorescent fluorescein (excitation/emission ~490/514 nm).
This conversion can be monitored via either fluorescence or absorbance, providing flexibility for various assay formats. FDGlu’s high sensitivity makes it ideal for detecting low levels of β-glucosidase activity in cell biology, enzymology, and microbiology research.
Key Features
- High Sensitivity – One of the most sensitive substrates for β-glucosidase detection
- Bright Green Fluorescence – Excitation/emission maxima at ~490/514 nm
- Cell-Permeant – Suitable for live-cell and fixed-cell assays
- Versatile Detection – Monitor via fluorescence microscopy, flow cytometry, fluorometry, or absorbance
- Flexible Applications – For both in vitro biochemical assays and live-cell enzyme activity studies
Applications
- β-glucosidase enzyme activity measurement in cell lysates or live cells
- Glycosidase activity profiling in biochemical research
- Enzyme kinetics and inhibitor screening
- Cell-based functional assays in microbiology and plant biology
- Live-cell fluorescence microscopy and flow cytometry assays
Specifications
| Parameter | Details |
|---|---|
| Catalog Number | F2881 (also known as F-2881) |
| Chemical Name | Fluorescein Di-β-D-Glucopyranoside |
| Quantity | 5 mg |
| Substrate Type | β-glucosidase substrate |
| Excitation/Emission | ~490/514 nm |
| Cell Permeability | Yes |
| Detection Method | Fluorescence or absorbance |
| Form | Solid |
| Storage Conditions | –5°C to –30°C |
| Shipping Conditions | Room temperature |
| Regulatory Status | Research Use Only (RUO) |
Invitrogen™ FDGlu is a high-performance β-glucosidase substrate that offers exceptional sensitivity, live-cell compatibility, and versatile detection capabilities. Whether for enzyme kinetics, functional assays, or cellular imaging, FDGlu provides a reliable and sensitive readout for β-glucosidase activity.