Thermo Scientific™ Oxoid™ Brilliant Green Bile Broth 2% (Dehydrated), 500g

Thermo Scientific™ Oxoid™ Brilliant Green Bile Broth 2% is a selective dehydrated culture medium designed for the detection and confirmation of coliform bacteria (coli-aerogenes group) in water, wastewater, food, milk, and dairy products. Formulated to meet international microbiological standards, this medium enables reliable identification of coliforms through lactose fermentation and gas production.

The combination of bile salts and brilliant green dye provides strong selective properties by inhibiting non-target microorganisms, ensuring accurate and reproducible results in quality control and microbiological testing laboratories.

Key Features

• Selective for Coliform Bacteria – Targets members of the coli-aerogenes group
• Reliable Detection Method – Gas production from lactose fermentation enables easy identification
• Strong Selective Agents – Bile salts and brilliant green inhibit unwanted organisms
• Optimized Formulation – Suppresses anaerobic lactose fermenters without affecting coliforms
• Dehydrated Format – Long shelf life and convenient preparation
• High Yield – Prepares up to 12.5 L of medium
• Compliant with International Standards – Suitable for regulated testing environments

Applications

• Water & Wastewater Testing
  • Detection of coliform contamination
• Food & Beverage Microbiology
  • Quality control in dairy, milk, and food products
• Environmental Monitoring
  • Hygiene and sanitation assessment
• Industrial Microbiology
  • Routine microbiological analysis in manufacturing
• Regulatory Testing Laboratories
  • Compliance with ISO, AOAC, and other standards

Technical Specifications

Principle of the Method

• Lactose fermentation by coliform bacteria produces gas, which serves as a key indicator of presence
• Bile salts inhibit Gram-positive organisms
• Brilliant green dye suppresses non-target Gram-negative bacteria
• The optimized formulation ensures selective growth of coliform organisms while reducing false positives

Unique Advantages

• Highly selective and reliable for coliform detection
• Easy-to-interpret results through visible gas production
• Widely accepted standard method medium
• Suitable for regulatory and compliance testing
• Trusted Oxoid™ formulation for consistent performance

Thermo Scientific™ Oxoid™ Brilliant Green Bile Broth 2% is a gold-standard selective medium for the detection and confirmation of coliform bacteria in environmental and food samples. Its optimized formulation, compliance with international standards, and ease of interpretation make it an essential tool for microbiology laboratories focused on quality control and safety testing.