Applied Biosystems™ Custom TaqMan™ Gene Expression Assay, FAM

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Specifications:

Application	Gene Expression, Multiplex qPCR
Storage Temperature	-15°C to -25°C
Product Type	PCR Amplification Kit	Forms	Liquid
Product Brand	Thermo Fisher Scientific™, Applied Biosystems™
Product Grade	Molecular Biology

Overview
Resource

Made-to-Order qPCR Assays for Gene Expression Analysis

Applied Biosystems™ Custom TaqMan™ Gene Expression Assays are made-to-order real-time PCR assays designed for quantitative gene expression analysis. These assays provide the custom version of Applied Biosystems™ predesigned TaqMan™ assays and are suitable when a specific target, transcript, gene variant, or sequence region requires a customized assay design.

Each assay includes a pair of unlabeled PCR primers and a sequence-specific TaqMan™ probe labeled with FAM dye at the 5′ end, plus a minor groove binder (MGB) and nonfluorescent quencher (NFQ) at the 3′ end. This chemistry supports high specificity, strong sensitivity, and accurate quantification of gene expression by real-time PCR.

Key Features

Feature	Description
Custom assay design	Made-to-order assay for user-specified gene expression targets
FAM-labeled probe	Includes a TaqMan™ MGB probe with FAM dye for real-time fluorescence detection
High specificity	MGB probe chemistry improves target specificity and helps reduce cross-reactivity
Sensitive detection	Suitable for low-expression genes and low-abundance targets
Accurate quantification	Enables reliable detection of small fold-changes in gene expression
Easy qPCR setup	Add cDNA and master mix; no melt curve analysis required
Pre-formulated assay mix	Supplied as a ready-to-use primer and probe assay mix
Sequence information provided	Assay sequences are provided with every custom assay ordered

Product Variants

Catalog Number	Assay Size	Quantity	Format
4331348	S	360 reactions	Made to order
4332078	M	750 reactions	Made to order
4332079	L	2,900 reactions	Made to order

Applications

Application Area	Use
Gene expression analysis	Quantitative measurement of mRNA expression levels
qPCR research workflows	Real-time PCR analysis using TaqMan™ probe-based chemistry
Low-abundance target detection	Sensitive quantification of targets expressed at low levels
Transcript analysis	Custom assay design for specific gene or transcript targets
Gene variant studies	Assay development for targets with high sequence similarity
Biomarker research	Quantification of expression changes in research samples
Validation studies	Confirmation of expression results from discovery workflows

Assay Format and Composition

Component	Description
PCR primers	2 unlabeled PCR primers
Probe	1 FAM dye-labeled TaqMan™ MGB probe
Probe quencher	Nonfluorescent quencher, NFQ
Probe chemistry	MGB probe for improved specificity
Final 1X primer concentration	900 nM each
Final 1X probe concentration	250 nM
Recommended master mix	TaqMan™ Fast Advanced Master Mix

Compatible Instruments

Applied Biosystems™ Real-Time PCR Systems
7500 System
7500 Fast System
7900HT System
StepOne™
StepOnePlus™
ViiA™ 7 System
QuantStudio™ 3
QuantStudio™ 5
QuantStudio™ 6 Flex
QuantStudio™ 7 Flex
QuantStudio™ 6 Pro
QuantStudio™ 7 Pro
QuantStudio™ 12K Flex

Technical Specifications

Parameter	Specification
Product Name	Applied Biosystems™ Custom TaqMan™ Gene Expression Assay, FAM
Product Line	TaqMan™
Product Type	Custom TaqMan™ Gene Expression Assay
Detection Dye	FAM
Probe Type	TaqMan™ MGB probe
Quencher	NFQ, nonfluorescent quencher
Assay Format	Pre-formulated primer and probe mix
S and M Size Concentration	20X assay mix
L Size Concentration	60X assay mix
Shipping Condition	Room temperature
Storage	Store at -15°C to -25°C
Unit Size	Each
Use Statement	For Research Use Only. Not for use in diagnostic procedures

Unique Advantages

Advantage	Benefit
Gold-standard TaqMan™ chemistry	Supports reliable probe-based qPCR performance
MGB probe design	Allows shorter probes with improved specificity
No melt curve requirement	Simplifies workflow compared with dye-based assays
Custom target flexibility	Useful when predesigned assays are not available or when a specific sequence is required
Bioinformatics-supported design	Helps improve specificity and minimize cross-reactivity
Multiple reaction sizes	Enables users to select an assay size based on project scale

Applied Biosystems™ Custom TaqMan™ Gene Expression Assays, FAM provide reliable made-to-order qPCR assays for quantitative gene expression research. With FAM-labeled TaqMan™ MGB probe chemistry, pre-formulated primers and probe, and compatibility with a wide range of Applied Biosystems™ real-time PCR systems, these assays are well suited for sensitive, specific, and accurate measurement of gene expression targets.