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AgraQuant® Total Aflatoxin ELISA Test Kit
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Analytes: Aflatoxin B1, Aflatoxin B2, Aflatoxin G1, Aflatoxin G2
Specifications:
| Application | ELISA-Based Assays, Food and feed safety testing, Mycotoxin Analysis | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Elisa Kit | Forms | Kit with Various components |
| Product Brand | Romer Labs | ||
| Product Grade | Analytical grade | ||
AgraQuant® Total Aflatoxin ELISA Test Kit is a quantitative enzyme-linked immunosorbent assay designed for accurate and reliable detection of total aflatoxins, including Aflatoxin B1, B2, G1, and G2. The kit is suitable for laboratories performing routine mycotoxin testing in food, feed, grain, and agricultural product quality control workflows.
When used with a compatible ELISA reader such as StatFax® or BioTek®, results can be directly interpreted and continuously documented. The assay supports parallel measurement of multiple samples, with incubation times as low as 15 minutes for up to 42 samples, helping laboratories improve throughput and reduce overall time-to-result.
Key Features
| Feature | Details |
|---|---|
| Quantitative ELISA Format | Provides measurable total aflatoxin results |
| Broad Aflatoxin Coverage | Detects Aflatoxin B1, B2, G1, and G2 |
| Multiple Sensitivity Options | Available in 1 ppb and 3 ppb detection limit formats |
| Fast Workflow | Incubation times as low as 15 minutes for up to 42 samples |
| Parallel Sample Testing | Suitable for routine high-throughput mycotoxin screening |
| Reader Compatible | Designed for use with StatFax® or BioTek® ELISA readers |
| Efficient Extraction Workflow | Up to 6 mycotoxins can be analyzed from one extraction using AgraQuant® ELISA workflows |
| Cold Storage | Stored at 2–8 °C to maintain kit performance |
Applications
AgraQuant® Total Aflatoxin ELISA Test Kit is suitable for:
- Total aflatoxin analysis
- Food and feed safety testing
- Grain and cereal quality control
- Agricultural commodity testing
- Mycotoxin monitoring programs
- Product testing laboratories
- Quantitative ELISA-based contaminant testing
- Routine screening of multiple samples
- Aflatoxin B1, B2, G1, and G2 detection
Product Specifications
| Specification | Details |
|---|---|
| Product Name | AgraQuant® Total Aflatoxin ELISA Test Kit |
| Analytes | Aflatoxin B1, Aflatoxin B2, Aflatoxin G1, Aflatoxin G2 |
| Technology / Application | ELISA |
| Result Type | Quantitative |
| Product / Environment | Product Testing |
| Quantitation Range | 1–20 ppb or 4–40 ppb, depending on kit format |
| Limit of Detection | 1 ppb or 3 ppb, depending on kit format |
| Storage Temperature | 2–8 °C |
| Detection Wavelength | 450 nm with 630 nm differential/reference filter |
Ordering Information
| Article Number | Alternate Code | Quantitation Range | Limit of Detection | Number of Wells | Analyte |
|---|---|---|---|---|---|
| 10002098 | COKAQ1000 | 4–40 ppb | 3 ppb | 96 | Aflatoxin B1, B2, G1, G2 |
| 10002099 | COKAQ1048 | 4–40 ppb | 3 ppb | 48 | Aflatoxin B1, B2, G1, G2 |
| 10002100 | COKAQ1100 | 1–20 ppb | 1 ppb | 96 | Aflatoxin B1, B2, G1, G2 |
| 10002101 | COKAQ1148 | 1–20 ppb | 1 ppb | 48 | Aflatoxin B1, B2, G1, G2 |
Materials Supplied
| Supplied Component |
|---|
| Antibody-coated microwells in a microwell holder |
| Non-coated dilution microwells |
| 5 or 6 vials of aflatoxin standards, depending on kit format |
| 1 bottle of aflatoxin conjugate |
| 1 bottle of substrate solution |
| 1 bottle of stop solution |
Materials Required but Not Supplied
| Required Material / Equipment |
|---|
| Romer Series II® Mill or equivalent |
| Blender or tightly sealing jar with lid |
| Balance, 400 g capacity |
| 100 mL graduated cylinder |
| 100% methanol, ACS grade, or 70% methanol |
| Distilled or deionized water for preparing 70% methanol |
| 84% acetonitrile |
| Container with minimum 125 mL capacity |
| Whatman No. 1 filter paper or equivalent |
| Filter funnel |
| MycoSep® 112 column for specific matrices |
| 8-channel and single-channel pipettes |
| 100 µL and 200 µL pipette tips |
| Timer |
| Wash bottle |
| Absorbent paper towels |
| 3 reagent boats for 8-channel pipettes |
| Microwell reader with 450 nm filter and 630 nm differential/reference filter |
Storage & Handling
Store the kit at 2–8 °C. Follow the product protocol for reagent preparation, sample extraction, incubation, washing, and result interpretation. Use appropriate laboratory safety procedures when handling methanol, acetonitrile, standards, conjugate, substrate, and stop solution.
Research / Testing Use Statement
For laboratory product testing use. Follow the manufacturer’s instructions and applicable laboratory quality procedures for sample preparation, assay execution, result interpretation, and documentation.
- Pack Size: 96 Tests Plate 48 Tests Plate
- Quantitation Range: 4 - 40 ppb 1 - 20 ppb