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Thermo Scientific™ MgCl2 (magnesium chloride) (25 mM), 5mL
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Store at -20°C
Specifications:
| Application | PCR | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ MgCl₂ (25 mM) solution is a high-purity, PCR-grade magnesium chloride reagent designed to meet the rigorous demands of modern molecular biology workflows. Magnesium ions (Mg²⁺) are essential cofactors in PCR reactions, playing a critical role in enzyme activity, primer annealing, and overall amplification efficiency.
This 25 mM solution is formulated for use with Taq DNA Polymerase and can be used to optimize magnesium concentration for maximum yield and specificity across a wide range of PCR-based applications. It is particularly valuable when working with complex templates or when precise control of ionic conditions is required.
Key Features
- Ready-to-Use Format: Supplied as a 25 mM liquid solution for convenience and accuracy.
- PCR-Grade Purity: Minimizes the risk of contamination to ensure reliable, reproducible results.
- Enhanced Reaction Control: Allows fine-tuning of magnesium levels for optimal enzyme performance.
- Broad Compatibility: Suitable for standard PCR, high-fidelity PCR, qPCR, and multiplex amplification.
Specifications
| Attribute | Details |
|---|---|
| Chemical Name | Magnesium Chloride (MgCl₂) |
| Concentration | 25 mM |
| Form | Liquid |
| Volume | 5 mL |
| Recommended Use | PCR reactions with Taq DNA Polymerase |
| Shipping Conditions | Wet or Dry Ice |
| Storage Temperature | –20°C |
Applications
- Routine PCR and endpoint analysis
- High-sensitivity and high-fidelity DNA amplification
- qPCR and RT-PCR optimization
- Molecular cloning workflows
- Mutation detection and genotyping
Thermo Scientific™ MgCl₂ (25 mM) offers researchers a dependable and flexible solution for optimizing PCR conditions. Its high quality and ease of use make it ideal for both standard and advanced amplification techniques. Whether you're troubleshooting reaction conditions or scaling up assays, this reagent supports robust, consistent performance in your molecular biology workflows.