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Applied Biosystems™ Absolute Q™ Universal DNA Digital PCR Master Mix (5X), 200 Reactions
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Specifications:
| Application | Digital PCR | ||
| Storage Temperature | 2-8°C | ||
| Product Type | PCR Master Mix | Forms | Lyophilized |
| Product Brand | Thermo Fisher Scientific™, Applied Biosystems™ | ||
| Product Grade | Molecular Biology | ||
The Absolute Q™ Universal DNA Digital PCR Master Mix (5X) is a highly optimized reagent system designed for use with the QuantStudio™ Absolute Q™ Digital PCR System. It enables robust, precise, and sensitive digital PCR (dPCR) performance across a broad range of applications, while offering convenience through compatibility with over 20 million predesigned Applied Biosystems™ TaqMan® Assays.
With 72-hour benchtop stability, high tolerance to inhibitors, and the ability to multiplex up to four targets per reaction, this master mix delivers exceptional performance for both research flexibility and workflow efficiency.
Key Features
- Universal compatibility – works with >20 million Applied Biosystems™ predesigned TaqMan® Assays.
- No need for custom assay design – use the same assay for both qPCR and dPCR.
- 72-hour room temperature stability – supports automation and extended walkaway time.
- Multiplexing capability – detect up to 4 targets per reaction.
- Inhibitor tolerance – ensures robust amplification even with complex samples.
- Seamless workflow – straightforward transition from qPCR to dPCR.
- Convenient storage – stable at 2–8 °C with a two-year shelf life.
Applications
- Gene expression analysis (cDNA recommended from SuperScript™ IV VILO™ Master Mix).
- Viral vector quantification for gene therapy workflows.
- Mutation detection in research studies.
- SNP genotyping for population and functional studies.
- Copy number variation (CNV) analysis.
- Microbial detection and quantification.
Simple Workflow
- Select a predesigned TaqMan® Assay.
- Combine the assay with the master mix and your DNA sample.
- Load into the MAP16 plate.
- Run on the QuantStudio™ Absolute Q™ Digital PCR System.
This streamlined process provides sensitive and reproducible dPCR results with minimal optimization.
Specifications
| Property | Value |
|---|---|
| Catalog Number | A72710 |
| Concentration | 5X |
| Format | Liquid |
| Volume | 400 μL |
| Reaction Format | 9 μL |
| No. of Reactions | 200 |
| Polymerase | Hot Start Taq DNA polymerase |
| Hot Start | Yes |
| Detection Method | Primer–probe (TaqMan chemistry) |
| Sample Types | DNA, cDNA |
| Compatible System | QuantStudio™ Absolute Q™ Digital PCR System, MAP16 plates |
| Applications | Digital PCR (dPCR), DNA quantitation, gene expression, SNP genotyping, microbial detection |
| PCR Method | Digital PCR |
| Reaction Speed | Fast or standard |
| Performance | High performance with GC-rich templates |
| Shipping Condition | 2–8 °C |
| Storage | Store at 2–8 °C; 2-year shelf life |
| Unit Size | Each (200 reactions) |
Ordering Information
| Catalog Number | Description | Quantity |
|---|---|---|
| A72710 | Absolute Q™ Universal DNA Digital PCR Master Mix (5X) | 1 × 400 µL (200 reactions) |
The Absolute Q™ Universal DNA Digital PCR Master Mix delivers unmatched accuracy, convenience, and flexibility for researchers performing digital PCR applications. With its universal assay compatibility, multiplexing capability, and long benchtop stability, it streamlines workflows and ensures consistent results in genomics, infectious disease research, oncology, and synthetic biology.