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Invitrogen™ Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 594, 1mg
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Specifications:
| Application | Western Blot, immunocytochemistry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Secondary Antibodies | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Invitrogen™ Goat anti-Mouse IgG (H+L), Alexa Fluor™ Plus 594, is a highly cross-adsorbed polyclonal secondary antibody designed for sensitive and specific detection of mouse primary antibodies. It recognizes both heavy and light chains of mouse immunoglobulins, and is cross-adsorbed against a broad panel of other species, significantly reducing background and cross-reactivity in multiplex fluorescence applications.
Conjugated to Alexa Fluor™ Plus 594, this antibody provides brilliant red fluorescence and is ideal for imaging applications including immunocytochemistry (ICC/IF), immunohistochemistry (IHC), and Western blotting (WB).
Key Features and Benefits
- Highly Cross-Adsorbed: Minimized cross-reactivity with bovine, goat, rabbit, rat, and human immunoglobulins
- Versatile Use: Suitable for ICC/IF, IHC, WB, and multicolor flow cytometry
- Bright Signal: Alexa Fluor™ Plus 594 dye ensures high signal-to-noise ratio and low background
- Stable Format: Supplied in proprietary buffer with preservatives for extended shelf life
- Excellent Specificity: Binds mouse IgG heavy chains and light chains of all mouse immunoglobulins
- Validated in Multiple Applications: Extensively referenced in scientific literature (200+ publications)
Applications & Recommended Dilutions
| Application | Suggested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000 |
| Immunocytochemistry (ICC) | 1–10 µg/mL |
| Immunofluorescence (IF) | 1–10 µg/mL |
Note: Optimal dilution should be determined empirically for each application and sample type.
Product Specifications
| Attribute | Detail |
|---|---|
| Host Species | Goat |
| Reactivity | Mouse |
| Immunogen | Mouse IgG (H+L chains) |
| Conjugate | Alexa Fluor™ Plus 594 |
| Excitation/Emission Max | 590/618 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Cross-Adsorption | Bovine, goat, rabbit, rat, and human IgGs |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 6.5 |
| Preservatives | 0.016% Methylisothiazolone, 0.016% Bromonitrodioxane |
| Storage Conditions | 4°C, protect from light |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Specificity and Handling Notes
- Specificity: Binds heavy chains of mouse IgG and light chains of all mouse immunoglobulins.
- Non-reactivity: Does not bind to mouse serum proteins or immunoglobulins from bovine, goat, rabbit, rat, or human.
- Handling Tip: Centrifuge briefly before use to remove protein aggregates and reduce background.
The Goat anti-Mouse IgG (H+L) Alexa Fluor™ Plus 594 secondary antibody (A32742) offers excellent specificity, reduced cross-reactivity, and exceptional brightness, making it ideal for researchers conducting fluorescent imaging, immunostaining, and multiplex analysis. Its high performance and wide validation base make it a trusted choice in fluorescence-based applications.