Invitrogen™ PureLink™ Microbiome DNA Purification Kit, 50 Preps

The Invitrogen™ PureLink™ Microbiome DNA Purification Kit is a high-performance nucleic acid extraction system designed for the rapid isolation of high-quality microbial and host DNA from complex biological and environmental samples. Utilizing advanced PureLink™ silica spin column technology combined with a powerful triple lysis approach, this kit ensures efficient recovery of DNA from a broad spectrum of microorganisms, including hard-to-lyse species such as Gram-positive bacteria.

Engineered for versatility and reliability, the kit effectively removes PCR inhibitors commonly found in challenging matrices such as stool and soil, enabling accurate downstream analysis. It is ideally suited for microbiome research, pathogen detection, and genomic studies requiring reproducible, high-yield DNA extraction.

Key Features

• Triple Lysis Technology (Heat, Chemical, Mechanical)
  Ensures efficient disruption of all microorganism types, including robust cell wall structures.
• Optimized for Complex Samples
  Reliable performance with difficult matrices such as stool, soil, and environmental samples.
• Efficient Inhibitor Removal
  Specialized cleanup buffer removes PCR inhibitors, improving downstream assay performance.
• High-Quality DNA Yield
  Produces purified DNA suitable for sensitive applications such as qPCR and next-generation sequencing.
• Broad Sample Compatibility
  Eliminates the need for multiple extraction kits across different sample types.
• Spin Column-Based Workflow
  Simple, reliable, and reproducible purification using silica membrane technology.
• Fast Processing Time
  Complete workflow in approximately 55 minutes, including sample homogenization.

Applications

Microbiome Research

• Human microbiome profiling (gut, skin, oral, vaginal)
• Microbial diversity and metagenomics studies
• Host–microbe interaction analysis

Molecular Diagnostics Research

• Detection of pathogenic bacteria
• Surveillance of antimicrobial resistance genes
• Infectious disease research (RUO)

Environmental & Food Testing

• Soil microbiome analysis
• Water and environmental monitoring
• Food safety microbiology

Genomics & Molecular Biology

• PCR and qPCR analysis
• Next-generation sequencing (NGS)
• Southern blotting and hybridization
• Nucleic acid labeling

Supported Sample Types

• Stool
• Urine
• Saliva
• Swabs (buccal, vaginal, skin, rectal, environmental)
• Transport media
• Growth media
• Microbial cultures
• Soil
• Food and environmental samples

Technical Specifications

Starting Material Capacity

Kit Contents

• S1 Lysis Buffer (40 mL)
• S2 Lysis Enhancer (5 mL)
• S3 Cleanup Buffer (12.5 mL)
• S4 Binding Buffer (45 mL)
• S5 Wash Buffer (Concentrated, 13 mL)
• S6 Elution Buffer (5 mL)
• PureLink Spin Columns (50 units)
• Collection Tubes (100 units)
• Bead Tubes (50 units)

Ordering Information

Workflow Overview

Sample Preparation → Mechanical Disruption (Bead Beating) → Triple Lysis (Heat + Chemical) → Inhibitor Removal → DNA Binding (Spin Column) → Wash → Elution → Downstream Analysis

Unique Advantages

• Comprehensive Microbial Coverage
  Efficient lysis ensures recovery from both Gram-positive and Gram-negative organisms.
• Accurate Microbiome Representation
  Minimizes bias, enabling true reflection of microbial diversity.
• Reduced Inhibitor Interference
  Improves PCR sensitivity and sequencing accuracy.
• Single-Kit Versatility
  Supports a wide range of biological and environmental sample types.
• Reliable Downstream Compatibility
  Optimized for PCR, qPCR, and NGS workflows.

The Invitrogen™ PureLink™ Microbiome DNA Purification Kit is a robust, versatile, and efficient solution for extracting high-quality DNA from complex microbiome samples. Its advanced lysis technology and inhibitor removal system ensure reliable results across a wide range of applications, making it an essential tool for modern microbiome research and molecular biology workflows.