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Applied Biosystems™ MagMAX™ Cell-Free DNA Isolation Kit
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Specifications:
| Application | Cell-Free DNA (cfDNA) Extraction | ||
| Storage Temperature | Room Temperature, 2-8°C | ||
| Product Type | Nucleic Acid Kits | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Applied Biosystems™ | ||
| Product Grade | Molecular Biology | ||
The Applied Biosystems™ MagMAX™ Cell-Free DNA Isolation Kit is a high-performance solution designed for the efficient isolation and enrichment of circulating cell-free DNA (cfDNA) from plasma, serum, urine, and other liquid samples. Leveraging advanced magnetic bead-based purification technology, this kit delivers high yields of pure, fragmented DNA, ideal for sensitive downstream applications.
Optimized for both manual and automated workflows, the kit supports scalable sample input volumes and is fully compatible with KingFisher™ magnetic particle processors, enabling high-throughput and reproducible nucleic acid extraction.
Key Features
- Efficient isolation of circulating cell-free DNA (cfDNA)
- Magnetic bead-based purification for high yield and consistency
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Compatible with:
- Manual extraction (magnetic stand)
- Automated platforms (KingFisher™ Duo Prime, KingFisher™ Flex)
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Flexible sample input volumes:
- 100 µL to 10 mL
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Fast workflow:
- Process 6–24 samples in ~40 minutes
- Includes Dynabeads™ MyOne™ Silane for optimized DNA binding
- Phenol-free extraction for safer handling
- Elution volume: 15–50 µL for concentrated DNA recovery
Applications
This kit is ideal for:
- Liquid biopsy workflows
- Cancer research and biomarker discovery
- Circulating tumor DNA (ctDNA) analysis
- Non-invasive disease monitoring
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Downstream applications including:
- Real-Time PCR (qPCR)
- Digital PCR (dPCR)
- Next-Generation Sequencing (NGS)
Technology Overview
The kit utilizes magnetic bead-based nucleic acid purification:
- cfDNA binds to silica-coated magnetic beads (Dynabeads™ MyOne™ Silane)
- Magnetic separation enables efficient washing and removal of contaminants
- DNA is eluted in low volumes, concentrating fragmented cfDNA
- The result is high-quality DNA suitable for sensitive molecular assays
This approach eliminates issues associated with column-based systems, such as clogging and inconsistent yields, especially in protein-rich samples like plasma.
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | MagMAX™ Cell-Free DNA Isolation Kit |
| Catalog Number | A29319 |
| Target | Cell-Free DNA (cfDNA) |
| Sample Type | Plasma, Serum, Urine, Liquid Samples |
| Technology | Magnetic Bead-Based |
| Input Volume | 100 µL – 10 mL |
| Elution Volume | 15–50 µL |
| Processing Time | ~40 minutes |
| Automation Compatibility | KingFisher™ Duo Prime, KingFisher™ Flex |
| Downstream Applications | qPCR, dPCR, NGS |
| Format | Kit |
| Shipping | Room Temperature |
Kit Components
- MagMAX™ Cell-Free DNA Magnetic Beads (Dynabeads™ MyOne™ Silane)
- Lysis/Binding Solution (125 mL)
- Wash Solution (100 mL)
- Elution Solution (5 mL)
Advantages
- High recovery of low-abundance cfDNA fragments
- Scalable workflow for small to large sample volumes
- Reduced risk of clogging and sample loss
- Automation-ready for high-throughput labs
- Consistent and reproducible results
- Ideal for liquid biopsy and precision medicine research
The Applied Biosystems™ MagMAX™ Cell-Free DNA Isolation Kit is a powerful and flexible solution for cfDNA extraction from liquid samples, delivering high-quality DNA for advanced molecular applications. Its combination of magnetic bead technology, scalability, and automation compatibility makes it an essential tool for modern genomics, oncology research, and liquid biopsy workflows.