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Invitrogen™ Goat anti-Human IgG Fc Secondary Antibody, HRP, 1mg
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Species Reactivity: Human
Host/Isotype: Goat / IgG
Class: Polyclonal
Type: Secondary Antibody
Immunogen: Gamma Immunoglobin Fc region
Specifications:
| Application | Western Blot, ELISA-Based Assays, Immunohistochemistry | ||
| Storage Temperature | -20°C | ||
| Product Type | Secondary Antibodies | Forms | Lyophilized |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Goat anti-Human IgG Fc Secondary Antibody, HRP is a high-quality polyclonal antibody raised in goat and conjugated to horseradish peroxidase (HRP) for sensitive immunodetection. This antibody specifically recognizes the Fc region of Human IgG, enabling accurate detection of primary antibodies in multiple immunoassays.
It is affinity-purified, tested across various applications, and validated with 71 published references, ensuring reproducible performance in research use. This antibody is particularly suitable for Western blotting, immunohistochemistry, and ELISA, where signal amplification and sensitivity are essential.
Applications & Recommended Dilutions
- Western Blot (WB): 1:500 – 1:20,000
- Immunohistochemistry (IHC): 1:500 – 1:20,000
- ELISA: 1:500 – 1:20,000
Note: Optimal dilutions should be determined by the end user for specific applications.
Product Specifications
| Parameter | Details |
|---|---|
| Species Reactivity | Human |
| Target | IgG Fc region |
| Class / Type | Polyclonal, Secondary Antibody |
| Host / Isotype | Goat / IgG |
| Immunogen | Gamma Immunoglobulin Fc region |
| Conjugate | HRP (Horseradish Peroxidase) |
| Form | Lyophilized (rehydrate with 1.1 mL deionized water) |
| Concentration | 1.0 mg/mL |
| Purification | Affinity chromatography |
| Buffer | PBS (pH 7.2) with 1% BSA, 0.1% ProClin 150 preservative |
| Storage Conditions | 4°C (short term); -20°C (long term, after reconstitution with 50% glycerol) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535594 |
Product-Specific Information
- Each batch undergoes ELISA sensitivity testing and immunoelectrophoresis for specificity.
- No reactivity observed against non-immunoglobulin serum proteins or light chains of human immunoglobulins.
- Lyophilized material is overfilled to ensure complete recovery upon reconstitution.
- Recommended to prepare fresh working dilutions daily.
Target Information
Anti-Human secondary antibodies are affinity-purified reagents with high specificity to human immunoglobulins. They are widely used for:
- Detection of primary antibodies in immunoassays.
- Signal amplification as multiple secondaries can bind to one primary antibody.
- Versatility, with HRP enabling chemiluminescent, chromogenic, or colorimetric detection systems.
The Invitrogen™ Goat anti-Human IgG Fc Secondary Antibody, HRP (Cat. A18817) is a highly specific, affinity-purified polyclonal antibody ideal for immunoassays requiring robust detection and signal amplification. Its broad validation, proven reproducibility, and flexible application range make it an indispensable reagent for Western blotting, immunohistochemistry, and ELISA involving human targets. Supplied in a lyophilized format with long-term stability, this antibody ensures consistent results and reliability for researchers in immunology, diagnostics development, and protein analysis.