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Invitrogen™ Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488, 1mg
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Specifications:
| Application | immunocytochemistry, Flow Cytometry, Immunohistochemistry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Secondary Antibodies | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Bright green fluorescence with high specificity and minimal cross-reactivity for sensitive immunodetection
The Invitrogen™ Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 is a high-performance fluorescently labeled secondary antibody designed for the sensitive and specific detection of mouse primary antibodies in various immunoassays. Conjugated to the bright, photostable Alexa Fluor™ 488 dye, this antibody is ideal for applications including immunocytochemistry (ICC/IF), immunohistochemistry (IHC), and flow cytometry (Flow).
This antibody is cross-adsorbed against human IgG and serum proteins to minimize non-specific binding and background signal, making it particularly effective in multiplex and multicolor staining where cross-reactivity can compromise data integrity.
Key Features
- Bright, Photostable Signal: Alexa Fluor™ 488 offers exceptional brightness and photostability, with an excitation/emission max of 499/520 nm, ideal for FITC channels.
- Cross-Adsorbed for Specificity: Minimizes cross-reactivity with human IgG and serum proteins, reducing background noise in complex samples.
- High Purity: Affinity-purified whole goat IgG provides consistent and reproducible performance.
- Wide Application Use: Optimized for ICC/IF, IHC, and flow cytometry.
- Low Background: Ideal for detecting low-abundance targets or performing co-staining in multiplex workflows.
- Trusted Format: Proven performance supported by 9,500+ citations and 86+ published figures.
Applications
- Immunocytochemistry (ICC/IF)
- Immunohistochemistry (IHC)
- Flow Cytometry (FACS)
- Multiplex Fluorescence Assays
- Fluorescent in situ hybridization (FISH)
- High-content screening and imaging
Recommended Dilutions
| Application | Suggested Dilution |
|---|---|
| ICC/IF | 1 µg/mL |
| IHC | Assay-dependent |
| Flow Cytometry | 1–10 µg/mL |
Dilution factors should be optimized based on sample type and detection system.
Technical Specifications
| Specification | Details |
|---|---|
| Catalog Number | A-11001 |
| Host Species | Goat |
| Target | Mouse IgG (H+L chains) |
| Antibody Type | Polyclonal, Whole Antibody |
| Conjugate | Alexa Fluor™ 488 |
| Excitation/Emission Max | 499/520 nm |
| Purity | Affinity-purified |
| Concentration | 2 mg/mL |
| Form | Liquid |
| Buffer | PBS, pH 7.5, 5 mM sodium azide |
| Cross-Adsorbed Against | Human IgG and serum |
| Storage | 4°C, protect from light |
| Shipping Conditions | Room temp (domestic); wet ice (international) |
| RRID | AB_2534069 |
| Regulatory Status | Research Use Only (RUO) |
Product Benefits
- High specificity for mouse IgG (H+L) with reduced non-specific staining
- Excellent performance in multicolor experiments and multiplexed fluorescence imaging
- Bright signal and low background ideal for low-expressing antigens
- Versatile use across fixed and live cell applications
The Invitrogen™ Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 provides superior sensitivity, minimal cross-reactivity, and robust performance for a wide range of fluorescence-based detection methods. Whether you're working with complex tissues, delicate cell cultures, or advanced multiplexing assays, this reagent ensures clear, reliable signal and confident data interpretation.