The Liofilchem® Meropenem+Phenylboronic Acid (MR+BO) Antimicrobial Susceptibility Discs are designed to detect Klebsiella pneumoniae carbapenemase (KPC) production and other class A beta-lactamase activity in Gram-negative bacteria, including Enterobacterales. These discs combine Meropenem with Phenylboronic Acid (PBA), an inhibitor of KPC enzymes, for reliable detection of carbapenem-resistant strains due to KPC production.
Key Features
- Specific Detection:
- Identifies KPC-producing bacteria by observing the synergistic effect of PBA in restoring meropenem activity.
- Dual Disc System:
- Includes separate discs for Meropenem (MR) and Meropenem+Phenylboronic Acid (MR+BO) for comparative zone diameter analysis.
- Accurate Results:
- A ≥5 mm increase in the zone of inhibition with MR+BO compared to MR alone confirms KPC activity.
- Ease of Use:
- Compatible with the standardized disc diffusion method on Mueller-Hinton agar.
- Clinical Relevance:
- Essential for guiding antimicrobial therapy in infections caused by carbapenem-resistant pathogens.
Applications
| Application | Description |
|---|
| Clinical Diagnostics | Detects KPC production in Gram-negative pathogens. |
| Antimicrobial Resistance Studies | Monitors the prevalence of KPC-producing bacteria in healthcare settings. |
| Pharmaceutical Research | Evaluates carbapenem+PBA combination therapies against resistant strains. |
Testing Procedure
- Preparation:
- Prepare a bacterial suspension to 0.5 McFarland standard.
- Spread the suspension uniformly on Mueller-Hinton agar plates.
- Application:
- Place the Meropenem (MR) and Meropenem+PBA (MR+BO) discs on the agar surface using sterile forceps.
- Incubation:
- Incubate plates at 35–37°C for 16–20 hours under aerobic conditions.
- Interpretation:
- Measure and compare the zones of inhibition for both discs:
- A zone diameter increase of ≥5 mm with MR+BO indicates KPC production.
Storage and Stability
| Condition | Temperature | Duration |
|---|
| Long-Term Storage | -20°C | Until expiration date |
| Short-Term Storage | 2–8°C | Up to 2 weeks |
| During Use | Room Temperature | Immediate use only |
Technical Specifications
| Feature | Details |
|---|
| Active Components | Meropenem (10 µg) and Meropenem+Phenylboronic Acid (10 µg + PBA) |
| Test Method | Disc diffusion |
| Compatible Media | Mueller-Hinton agar |
| Incubation Conditions | 35–37°C for 16–20 hours |
| Target Pathogens | KPC-producing Gram-negative bacteria |
| Shelf Life | As per expiration date on packaging |
| Pack Sizes | 50 or 100 discs per pack |
Quality Control Data
| Control Organism | Expected Results |
|---|
| Escherichia coli ATCC® 25922 | MR: ≥23 mm; MR+BO: Similar |
| Klebsiella pneumoniae (KPC-positive strain) | MR: Reduced zone; MR+BO: Zone diameter increased by ≥5 mm |
| Pseudomonas aeruginosa ATCC® 27853 | MR: ≥18 mm; MR+BO: Similar |
| Staphylococcus aureus ATCC® 29213 | Not applicable (Gram-positive control) |
- Positive Controls: Confirm detection of KPC-producing strains.
- Negative Controls: Ensure no false-positive results in non-KPC producers.
Interpretation of Results
| Zone of Inhibition Comparison | Interpretation |
|---|
| MR zone = MR+BO zone | Absence of KPC production. |
| MR zone ≥5 mm smaller than MR+BO zone | Presence of KPC-producing bacteria. |
Comparison with Other Disc-Based Tests
| Test Disc | Recommended Use |
|---|
| Meropenem+Phenylboronic Acid (MR+BO) | Specific detection of KPC beta-lactamase producers. |
| Meropenem+EDTA (MR+ED) | Detection of metallo-beta-lactamase (MBL) producers. |
| Meropenem+Cloxacillin (MR+CL) | Detection of AmpC beta-lactamase producers. |
| Carbapenemase Combination Discs | General detection of carbapenemase-mediated resistance. |
Advantages
| Feature | Benefit |
|---|
| Dual Testing System | Differentiates KPC resistance from other carbapenemases. |
| High Sensitivity and Specificity | Reduces false-positive and false-negative results. |
| Ease of Use | Simplified setup minimizes errors and time. |
| Standards Compliance | Adheres to global antimicrobial susceptibility testing guidelines. |
Conclusion
The Liofilchem® Meropenem+Phenylboronic Acid (MR+BO) Antimicrobial Susceptibility Discs offer a reliable and precise method for detecting KPC-mediated carbapenem resistance in Gram-negative bacteria. Their high sensitivity, specificity, and adherence to international testing standards make them essential for diagnostic laboratories and resistance monitoring programs.
http://www.labmartgh.com/shop/product/9176-liofilchem-r-meropenem-phenylboronic-acid-mr-bo-antimicrobial-susceptibility-discs-28281
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