Liofilchem® Campylobacter Skirrow Supplement, 10 x 10 mL

Liofilchem® Campylobacter Skirrow Supplement is a selective antibiotic mixture designed to facilitate the isolation of Campylobacter species from clinical, food, and environmental samples.

Developed from the original formulation by M.D. Skirrow (1977), this supplement provides a selective environment for the growth of Campylobacter jejuni and related species while effectively inhibiting competing Gram-positive and Gram-negative microorganisms.

When used with Campylobacter Agar Base (ref. 611007 / 621007) and Campylobacter Growth Supplement (ref. 81050), the medium achieves optimal selectivity and recovery of Campylobacter spp. under microaerophilic incubation conditions at 42 °C.

Key Features

• Classic Skirrow formulation for reliable Campylobacter isolation.
• Selective antibiotic combination—effective inhibition of non-target bacteria.
• Supports growth of Campylobacter jejuni and C. coli.
• Freeze-dried format: simple reconstitution, reproducible results.
• Each vial sufficient for 500 mL of medium.
• Ideal for clinical, veterinary, and food microbiology laboratories.

Composition

Principle of the Method

The Campylobacter Skirrow Supplement introduces a balanced antibiotic system that selectively suppresses competing flora, creating conditions favorable for the growth of Campylobacter jejuni and related species.

• Polymyxin B suppresses Gram-negative bacteria.
• Trimethoprim provides broad bacteriostatic activity against both Gram-positive and Gram-negative organisms.
• Vancomycin inhibits Gram-positive cocci.

This selective combination allows rapid and efficient isolation of Campylobacter spp. from mixed microbial samples incubated under microaerophilic conditions.

Preparation and Use

1. Aseptically reconstitute one vial of Campylobacter Skirrow Supplement with 2 mL of sterile distilled water.
2. Mix gently until dissolved (avoid foaming).
3. Add the entire contents (2 mL) aseptically to 500 mL of autoclaved Campylobacter Agar Base (45–50 °C) supplemented with:
   • 5–7% lysed horse blood, and
   • 1 vial of Campylobacter Growth Supplement (ref. 81050).
4. Mix carefully and pour into sterile Petri dishes.

Quality Control

Appearance: white freeze-dried powder

Incubation conditions: 48 h at 42 °C under microaerophilic atmosphere

Storage and Stability

• Store at 2–8 °C in the original, sealed packaging.
• Protect from moisture and light.
• Shelf life: valid until the expiry date indicated on the label (typically 4 years).
• Discard if discolored or contaminated.

Safety and Handling

• Contains antibiotics; handle with care.
• For in vitro diagnostic use only.
• Use by trained laboratory professionals under proper aseptic and biosafety procedures.
• Refer to the Safety Data Sheet (SDS) for detailed handling information.
• Dispose of materials in accordance with local biohazard regulations.

Ordering Information

Liofilchem® Campylobacter Skirrow Supplement provides a well-established antibiotic formulation that ensures selective recovery of Campylobacter spp., particularly C. jejuni and C. coli, from diverse sample types. When used with Campylobacter Agar Base and Growth Supplement, it delivers reproducible selectivity, effective suppression of background flora, and reliable diagnostic performance for clinical, food safety, and veterinary microbiology laboratories.