Liofilchem® Campylobacter Blaser Wang Supplement, 10 x 10 mL

Liofilchem® Campylobacter Blaser Wang Supplement is a selective antibiotic supplement developed for the isolation of Campylobacter species, including C. jejuni and C. coli, from clinical, food, and environmental samples.

This formulation is a modified version of Skirrow’s medium, enriched with Amphotericin B and Cephalothin, to provide enhanced selectivity and antifungal activity. When used with Campylobacter Agar Base (ref. 611007 / 621007) and Campylobacter Growth Supplement (ref. 81050), it forms a complete selective medium optimized for the growth of Campylobacter spp. under microaerophilic conditions.

Key Features

• Highly selective additive based on the Blaser-Wang modification of Skirrow’s formulation.
• Inhibits competing flora, including Gram-positive bacteria, Gram-negative enterics, and fungi.
• Promotes rapid recovery of Campylobacter jejuni and related species.
• Ready-to-use freeze-dried format—easy reconstitution and consistent quality.
• Each vial sufficient for 500 mL of medium.
• Validated for diagnostic and food microbiology laboratories.

Composition

Principle of the Method

The Campylobacter Blaser Wang Supplement enhances the selectivity of culture media used for isolating Campylobacter spp. The combination of antibiotics and antifungal agents suppresses competing microorganisms while allowing the growth of Campylobacter jejuni, C. coli, and related species under microaerophilic incubation (5–10% O₂, 10% CO₂).

• Polymyxin B inhibits Gram-negative contaminants.
• Trimethoprim interferes with nucleic acid synthesis, inhibiting Gram-positive and Gram-negative bacteria.
• Cephalothin and Vancomycin target Gram-positive flora.
• Amphotericin B prevents fungal contamination.

Preparation and Use

1. Reconstitute one vial of Campylobacter Blaser Wang Supplement with 4 mL of a 1:1 mixture of sterile distilled water and ethanol.
2. Shake gently until completely dissolved, avoiding foam formation.
3. Add the reconstituted solution aseptically to 500 mL of autoclaved Campylobacter Agar Base (45–50 °C) supplemented with:
   • 10% defibrinated ram’s blood or 5–7% lysed horse blood
   • 1 vial of Campylobacter Growth Supplement (ref. 81050)
4. Mix carefully and pour into Petri dishes.

Quality Control

Appearance: orange freeze-dried product

Incubation conditions: 48 h at 37 °C in a microaerophilic atmosphere

Storage and Stability

• Store at 2–8 °C in original packaging.
• Protect from humidity and light.
• Shelf life: Valid until expiry date indicated on the label (typically 4 years).
• Discard if product shows discoloration or deterioration.

Safety and Handling

• Contains antibiotics and antifungal agents—handle with care.
• For in vitro diagnostic use only.
• Use by trained laboratory professionals.
• Refer to the Safety Data Sheet (SDS) before use.
• Dispose of used materials in accordance with national biosafety regulations.

Ordering Information

Liofilchem® Campylobacter Blaser Wang Supplement provides a robust antibiotic-antifungal formulation optimized for the selective isolation of Campylobacter spp. from complex samples. Its combination of Polymyxin B, Trimethoprim, Cephalothin, Amphotericin B, and Vancomycin ensures inhibition of competing flora and improved recovery of fastidious Campylobacter strains. When used with Campylobacter Agar Base and Growth Supplement, it delivers reproducible, high-quality results ideal for clinical, food safety, and veterinary microbiology laboratories.