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Thermo Scientific™ Imject™ mcKLH Subunits, High Purity Research Grade, 20mg
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Specifications:
| Application | Protein Biology, Antibody Production and Purification | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Immunization Adjuvants | Forms | Solid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ Imject™ mcKLH Subunits, High Purity Research Grade is a purified keyhole limpet hemocyanin carrier protein designed for hapten conjugation, immunogen preparation, antibody production, vaccine research, and immunological studies.
Keyhole limpet hemocyanin is one of the most widely used carrier proteins for improving the immunogenicity of small antigens, including peptides, haptens, and other low-molecular-weight molecules. Because many small molecules are poorly immunogenic on their own, conjugation to KLH helps generate stronger immune responses in antibody production workflows.
Imject™ mcKLH is derived from mariculture-grown keyhole limpets, Megathura crenulata, supporting a more sustainable supply source while helping maintain product consistency. The material is purified and lyophilized for use in aqueous conjugation workflows and is suitable for research applications requiring a highly immunogenic carrier protein.
Key Features
- High-purity mcKLH carrier protein
- Designed for hapten conjugation and immunogen preparation
- Suitable for polyclonal and monoclonal antibody production
- Highly immunogenic carrier protein for small antigens and peptides
- Derived from mariculture-grown keyhole limpets
- Supports multiple conjugation methods, including EDC, NHS-ester, maleimide, and glutaraldehyde crosslinking
- Contains multiple reactive groups for hapten coupling
- Suitable for vaccine research, immune response studies, and antigen presentation workflows
- Supplied as a 20 mg research-grade product
- For Research Use Only
Technical Specifications
| Parameter | Details |
|---|---|
| Product Name | Thermo Scientific™ Imject™ mcKLH Subunits, High Purity Research Grade |
| Catalog Number | 77649 |
| Quantity | 20 mg |
| Product Type | Carrier protein for hapten conjugation |
| Protein Type | Keyhole limpet hemocyanin subunits |
| Source | Mariculture-grown keyhole limpet, Megathura crenulata |
| Molecular Mass | Aggregates of 350 and 390 kDa subunits; approximately 4.5 × 10⁵ to 1.3 × 10⁷ Da |
| Form | Lyophilized |
| Solubility | Forms a clear to turbid blue solution in PBS or Milli-Q water |
| Recommended Reconstitution | Approximately 10 mg/mL in PBS or Milli-Q water, depending on protocol |
| Compatible Conjugation Methods | EDC, NHS-ester, maleimide, glutaraldehyde |
| Storage Conditions | 4°C, refrigerated |
| Freeze Guidance | Do not freeze |
| Shipping Condition | Ambient |
| Regulatory Status | For Research Use Only |
| Main Applications | Antibody production, immunogen preparation, vaccine research, immunological assays |
Applications
| Application Area | Typical Use |
|---|---|
| Hapten Conjugation | Coupling of small antigens, peptides, or haptens to improve immunogenicity |
| Immunogen Preparation | Preparation of antigen-carrier conjugates for immune response generation |
| Antibody Production | Generation of polyclonal or monoclonal antibodies in animal models |
| Vaccine Research | Antigen presentation and preclinical vaccine development studies |
| Immunology Research | Study of immune activation and antigen-specific responses |
| T-Cell Activation Studies | Use in workflows involving antigen-presenting cells and T-cell mediated responses |
| Cancer Immunotherapy Research | Carrier protein support in tumour antigen and cancer vaccine studies |
| Inflammation and Autoimmune Research | Immune stimulation in experimental disease models |
Conjugation and Crosslinking Options
Imject™ mcKLH can be used with several common crosslinking strategies depending on the functional group present on the antigen or hapten.
| Conjugation Method | Suitable For | Typical Buffer / Notes |
|---|---|---|
| EDC Crosslinking | Carboxyl-containing haptens or peptides | Commonly performed in MES buffer |
| NHS-Ester Crosslinking | Primary amine coupling | Commonly performed in phosphate buffer |
| Maleimide Crosslinking | Sulfhydryl-containing or cysteine-terminal peptides | Provides targeted thiol-based coupling |
| Glutaraldehyde Crosslinking | General amine-based conjugation | Useful for high antigen loading, but optimization is recommended |
Workflow Summary
A typical mcKLH conjugation and immunization workflow may include:
- Reconstitute mcKLH in PBS, Milli-Q water, or the recommended buffer.
- Select the appropriate crosslinking chemistry based on the antigen or hapten functional group.
- Combine the antigen or hapten with mcKLH under optimized conjugation conditions.
- Purify or desalt the conjugate where required.
- Mix the conjugate with a suitable adjuvant for immunization studies.
- Administer according to the selected research protocol.
- Monitor antibody response and screen using methods such as ELISA, Western blot, or immunohistochemistry.
Storage and Handling Notes
Imject™ mcKLH Subunits should be stored refrigerated at 4°C upon receipt. The product should not be frozen, as freezing may affect solubility and structural integrity.
Recommended handling considerations include:
- Store at 4°C in the original container
- Do not freeze
- Avoid excessive agitation during reconstitution
- Use suitable sterile buffers for preparation
- Prepare conjugates according to the selected crosslinking chemistry
- Use appropriate personal protective equipment during handling
- Follow good laboratory practice and institutional safety procedures
Related Products
| Related Product | Typical Use |
|---|---|
| Imject™ mcKLH in PBS | KLH carrier protein supplied in phosphate-buffered saline |
| Imject™ mcKLH in MES Buffer | KLH carrier protein optimized for EDC conjugation workflows |
| Imject™ EDC mcKLH Spin Kit | Ready-to-use kit for EDC-mediated hapten conjugation |
| Imject™ Freund’s Complete Adjuvant | Primary immunization support |
| Imject™ Freund’s Incomplete Adjuvant | Booster immunization support |
Thermo Scientific™ Imject™ mcKLH Subunits, High Purity Research Grade, Catalog #77649 is a high-quality KLH carrier protein designed for hapten conjugation, immunogen preparation, antibody production, and vaccine research. With strong immunogenic properties, broad compatibility with common crosslinking methods, and a sustainable mariculture-derived source, it provides a reliable carrier protein solution for advanced immunology and antibody development workflows.