Proteintech™ Ki-67 Monoclonal Antibody (1B9H2), 150 µL

The Proteintech Ki-67 Monoclonal Antibody (Clone 1B9H2) is a highly specific antibody designed for the detection of Ki-67 (MKI67), a well-established nuclear marker of cell proliferation. Ki-67 is expressed in all active phases of the cell cycle (G1, S, G2, and M phases) and is absent in resting cells (G0 phase), making it a critical biomarker for evaluating cellular proliferation and tumor growth.

This antibody is validated for multiple applications including immunohistochemistry (IHC), immunofluorescence (IF/ICC), flow cytometry (intracellular), and ELISA, providing flexibility across a wide range of research workflows in oncology, cell biology, and pathology.

Key Features

• Specific for Ki-67 (MKI67) proliferation marker
• Monoclonal antibody clone 1B9H2
• Reactivity: Human
• Validated applications:
  • Immunohistochemistry (IHC): 1:1,000–1:5,000
  • Immunofluorescence (IF/ICC): 1:1,000–1:4,000
  • Flow Cytometry (Intracellular): 0.2 µg/10⁶ cells
  • ELISA
• Detects nuclear Ki-67 expression in proliferating cells
• Supplied as unconjugated liquid antibody
• Purified using Protein G
• Stable formulation in PBS with glycerol and sodium azide

Applications

This antibody is ideal for:

• Assessment of tumor proliferation index (Ki-67 index)
• Immunohistochemical analysis of cancer tissues
• Immunofluorescence imaging of proliferating cells
• Flow cytometry-based cell cycle and proliferation studies
• Research in:
  • Breast cancer prognosis
  • Head and neck cancers
  • Cell cycle regulation
  • Tumor progression and aggressiveness

Technical Specifications

Target Information

Ki-67 (MKI67) is a nuclear protein strictly associated with cell proliferation. It is present during all active phases of the cell cycle but absent in quiescent cells, making it one of the most reliable markers for determining the growth fraction of a cell population.

Ki-67 expression increases significantly during the S phase, and its detection is widely used to assess tumor aggressiveness and prognosis. High Ki-67 levels are often associated with poor clinical outcomes in cancers such as breast cancer and head and neck tumors.

Advantages

• Gold-standard marker for cell proliferation analysis
• Broad application compatibility (IHC, IF, FC, ELISA)
• High specificity and reproducibility
• Suitable for tumor grading and research applications
• Reliable monoclonal consistency

Ordering Information

The Proteintech Ki-67 Monoclonal Antibody (1B9H2) is a powerful and widely used tool for assessing cell proliferation and tumor growth dynamics. Its validated performance across multiple platforms makes it an essential reagent for oncology research, histopathology studies, and cell cycle analysis.