Liofilchem® Tryptose Phosphate Broth, 500g

Liofilchem® Tryptose Phosphate Broth is a nutrient-rich liquid culture medium formulated for the growth and maintenance of fastidious microorganisms from clinical and environmental specimens.

It supports a wide range of bacteria, including Streptococcus spp., Neisseria spp., and other nutritionally demanding organisms.

This medium provides excellent recovery and proliferation of fastidious species and is compliant with international microbiological standards for clinical and sanitary testing.

Key Features

• Broad-spectrum growth support: Promotes the cultivation of Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, and Escherichia coli.
• High nutritional content: Enzymatic digest of casein provides amino acids, nitrogen, and carbon sources essential for microbial growth.
• Buffered system: Disodium phosphate maintains a stable pH (7.3 ± 0.2 at 25°C) throughout incubation.
• Energy source: Glucose supports rapid bacterial metabolism and growth.
• Osmotic balance: Sodium chloride maintains osmotic equilibrium for sensitive strains.
• Optional semi-solid modification: Addition of 1–2 g/L agar supports anaerobic growth in the lower zone of the medium.
• Excellent clarity: Ready-to-use medium appears clear to slightly opalescent, light amber in color.

Principle

The enzymatic digest of casein provides a complete range of nutrients—amino acids, vitamins, and minerals—while glucose serves as an energy source. Sodium chloride maintains osmotic stability, and disodium phosphate acts as a buffering agent to maintain a neutral environment conducive to microbial growth.

Typical Formula (g/L)

Preparation

1. Suspend 29.5 g of powder in 1 L of deionized or distilled water.
2. Heat and stir until completely dissolved.
3. Dispense into suitable tubes or flasks.
4. Sterilize by autoclaving at 121°C for 15 minutes.
5. For enhanced anaerobic conditions, add 1–2 g/L of agar.

Directions for Use

1. Inoculate the broth with the specimen.
2. Incubate at 35 ± 2°C for 18–48 hours.
3. Observe for turbidity, indicating microbial growth.

Quality Control

Inoculum: ≤100 CFU

Incubation Conditions: 18–48 h at 35 ± 2°C

Product Specifications

Applications

• Clinical Microbiology: Cultivation of fastidious pathogens from clinical samples.
• Food & Water Testing: Isolation of fastidious organisms from environmental and sanitary samples.
• Pharmaceutical & Cosmetic Quality Control: Routine microbial monitoring.
• Research & Diagnostic Laboratories: General-purpose enrichment medium for microbial studies.

Safety & Precautions

• For in vitro diagnostic use only.
• Handle under aseptic conditions by trained personnel.
• Refer to the product Safety Data Sheet (SDS) for safe handling and disposal.
• Dispose of waste according to national and local biosafety regulations.

References

• Harmon, S.M., et al. (1995). FDA Bacteriological Analytical Manual, 8th ed., AOAC International.
• MacFaddin, J.D. (1985). Media for Isolation, Cultivation, Identification and Maintenance of Medical Bacteria, Vol. 1, Williams & Wilkins.
• Ginsberg, Gold & Jordan (1955). Proc. Soc. Exp. Biol. Med. 89:66.

Liofilchem® Tryptose Phosphate Broth provides an optimized formulation for the growth of fastidious microorganisms from clinical and non-clinical specimens.

With its balanced nutrient composition, buffered pH, and proven performance across key reference strains, this medium ensures reliable cultivation results for laboratories focused on diagnostics, food safety, and microbiological research.