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Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | -20°C, 2-8°C | ||
| Product Type | Elisa Kit | Forms | Kit with Various components |
| Product Brand | Mabtech | ||
| Product Grade | Molecular Biology | ||
The ELISpot Path: CMV Human IFN-γ Kit is a ready-to-use solution for the enumeration of human IFN-γ-secreting cells in response to cytomegalovirus (CMV) peptides.
This kit provides all reagents necessary for a reliable and sensitive enzyme-linked immunospot (ELISpot) assay, enabling precise functional analysis of T-cell immune responses against CMV antigens.
The kit contains a pre-coated ELISpot plate, matched monoclonal antibodies against human IFN-γ, and a CMV peptide pool containing both CD4⁺ and CD8⁺ T-cell epitopes. It is optimized for rapid setup and consistent detection of cytokine-secreting cells.
Key Features
- Comprehensive kit design: Includes all essential components—pre-coated plate, detection antibodies, enzyme substrate, CMV peptide pool, and positive control (anti-CD3 mAb).
- Matched monoclonal antibodies: Based on high-specificity IFN-γ mAb pair (1-D1K / 7-B6-1, ALP).
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Validated CMV peptide pool: Contains 42 synthetic peptides derived from CMV pp50, pp65, IE1, IE2, and envelope glycoprotein B.
- 28 peptides are MHC class I-restricted (CD8 epitopes).
- 14 peptides are MHC class II-restricted (CD4 epitopes).
- Positive control included: Anti-CD3 mAb (clone CD3-2) for assay verification.
- Sensitive and quantitative: Enables enumeration of cytokine-secreting T cells at the single-cell level.
- Extended stability: Shelf life of ≥ 18 months from receipt when stored correctly.
Kit Components
| Component | Description |
|---|---|
| Pre-coated ELISpot Plate | 96-well plate (strip format), pre-coated with anti-human IFN-γ mAb (clone 1-D1K) |
| Detection Antibody | Anti-human IFN-γ mAb (clone 7-B6-1) conjugated to alkaline phosphatase (ALP) |
| Substrate | BCIP/NBT-plus chromogenic substrate for ELISpot development |
| Positive Control | Anti-CD3 mAb (CD3-2) |
| Peptide Pool | 42 CMV-derived peptides (pp50, pp65, IE1, IE2, gB; CD4 + CD8 epitopes) |
| User Manual | Detailed instructions for assay setup and analysis |
Technical Specifications
| Parameter | Specification |
|---|---|
| Analyte | Human Interferon γ (IFN-γ) |
| Assay Principle | Enzyme-linked immunospot (ELISpot) |
| Reactivity | Human |
| Detection Antibody | HRP-conjugated or ALP-conjugated anti-IFN-γ (clone 7-B6-1) |
| Plate Coating Antibody | Anti-human IFN-γ (clone 1-D1K) |
| Peptide Pool | 42 CMV peptides (28 MHC I, 14 MHC II) |
| Shelf Life | ≥ 18 months from date of receipt |
| Shipping Conditions | Ambient temperature |
| Storage Conditions | 4–8 °C for antibodies, conjugate, and substrate; ≤ –20 °C for peptide pool; ambient for plates |
Applications
- Enumeration of IFN-γ-secreting T cells in response to CMV peptides
- Evaluation of CMV-specific cellular immunity in research studies
- Vaccine development and immunotherapy response monitoring
- Functional assays in transplantation and infectious disease research
- Cytokine-secreting cell profiling in human PBMCs or isolated T cells
Safety and Regulatory Information
- Intended Use: For Research Use Only (RUO). Not for diagnostic or therapeutic applications.
- Storage: Store per instructions above; do not freeze pre-coated plates.
- Shelf Life: Minimum of 18 months when stored as recommended.
The ELISpot Path: CMV Human IFN-γ Kit (3420-2AST-P2-1) provides a reliable, ready-to-use platform for analyzing CMV-specific T-cell responses through IFN-γ secretion measurement.
With pre-optimized components, validated monoclonal antibody pairs, and a comprehensive CMV peptide pool, this kit ensures consistent performance, rapid setup, and precise enumeration of functional immune cells in human samples.